Total selenium and organic selenium content of different winter wheat (Triticum aestivum L.) shoots and seeds

Abstract

In the course of the research we determined selenium and dry-matter content of 35 winter wheat shoots and 35 wheat seeds. The selenium content of the preparation plant probes was measured by spectrofluorimetric determination (λexcitation=380 nm, λemission=519 nm) of the resulted piaz-selenol complex. It was established that between the selenium content of the wheat shoots and wheat seed the correlation coefficient was 0.36 at p=0.05 level which indicates a medium close association. Similarly, there was a medium close association between selenium content of the wheat shoots calculated on dry-matter basis and total selenium content of the wheat seeds, with a correlation coefficient of 0.40 at p=0.02 level. After we determined the selenium as well as the seleno-methionine content of 44 winter wheat seeds samples by ion exchange column chromatography and high performance liquid chromatography, the organic selenium content was calculated. Very close correlation was established between selenium, seleno-methionine, and organic selenium content calculated from seleno-methionine content (the correlation coefficient was between 0.92 and 0.99 at p=0.01 level). The correlation between the seleno-methionine content of wheat shoots and seeds was very low (r=0.23).