Növényvédő szerek in vitro szemirritációs vizsgálata izolált csirkeszemen
Keywords:
in vitro, isolated chicken eye, eye irritation, pesticideAbstract
The toxicological studies for registration of pesticides are mostly based on mammals. Albino rabbits are used in the generally accepted eye irritation study developed by Draize et al. in 1944. Several various in vitro tests have been developed to replace in vivo eye irritation testing. One of these alternative methods is the Isolated Chicken Eye test. In the present study four different pesticides were screened for irritation potential by the Isolated Chicken Eye test. The in vitro results were compared with the in vivo data from material safety data sheet. In general a good correlation was found between in vitro results from ICE test and reported data based on the Draize eye test. The present form of the ICE test can be proposed as a screening method for the registration of pesticides, therefore the number of test animals can be reduced.
References
Draize, J. H., Woodard, G. and Calvery, H. O. 1944. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. Journal of Pharmacology and Experimental Therapeutics. 82. 377–390. https://doi.org/10.1016/S0022-3565(25)08751-8
OECD (Organisation for Economic Co-operation and Development) 2009. Test Guideline 438. OECD Guideline for the Testing of Chemicals. Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants.
Russell, W. M. S. and Burch, R. L. 1959. The Principles of Humane Experimental Technique, Methuen, London.
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Copyright (c) 2016 Kormos Éva, Buda István, Szabó Rita, Lehel József, Budai Péter
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