Növényvédő szerek primer szemirritációs vizsgálata EpiOcularTM szövetmodell alkalmazásával

Abstract

The in vivo Draize-test, which is the based on the OECD 405 guideline, is one of the most criticized in vivo methods, because of the subjective nature of the clinical observations and injuries of the test animals. Nowadays, several in vitro tests are available which can be partly or totally replaced the in vivo eye irritation testing depending on the circumstances. The EpiOcular™ model is one of these alternative methods. Five different agrochemicals were examined with this method and the irritation potential of the test items were predicted by measurement of their cytotoxic effect, as reflected in the MTT assay, on the EpiOcular™ tissue. Four test items showed irritation potential and one test item did not show irritation potential out of five examined test items. Compared these results with the available information about in vivo data of the tested agrochemicals, all test items are corresponded to them.