Peszticidek szemirritációs vizsgálata EpiOcularTM szövet modell alkalmazásával

Abstract

Draize-test is one of the most criticized in vivo methods, because of the injuries of the test animals and subjective nature of the test in recording the results. Nowadays, several in vitro tests are available which can be partly or totally replaced the in vivo eye irritation testing depending on the circumstances. The EpiOcular™ model is part of these alternative methods. Seven different pesticides were examined with this method and the irritation potential of the test item may be predicted by measurement of its cytotoxic effect, as reflected in the MTT assay, on the EpiOcular™ tissue. Four test items showed irritation potential and three test items did not show irritation potential out of seven examined test items. Compared to the results of these in vitro eye irritation studies with the available information about in vivo data of the tested pesticides, five test items are corresponded to them. However, two test items showed false positive result based on the data performed on experimental animals. However, false positive results in this context are not critical, because all test chemicals that come out positive would be subsequently tested with other adequately validated in vitro method(s) for the determination of final classification.