Examination of eye irritancy by using two alternative methods

Authors

  • Judit Tavaszi University of Pannonia, Georgikon Faculty, Department of Hygiene, Institute of Plant Protection H-8361 Keszthely, P. O. Box 71, Hungary
  • Éva Kormos University of Pannonia, Georgikon Faculty, Department of Hygiene, Institute of Plant Protection H-8361 Keszthely, P. O. Box 71, Hungary
  • Ágnes Pálovics Central Agricultural Office, Directorate of Plant Protection, Soil Conservation and Agri-environment H-1118 Budapest, 141-145. Budaörsi út, Hungary

Keywords:

chorioallantoic membrane, in vitro, rabbit, Draize-test, HET-CAM test, MTT-Assay

Abstract

Agrochemicals must undergo numerous toxicological tests before registration. To get knowledge about eye irritation, nowadays only the in vivo Draize-test is accepted, which is one of the most criticized methods because of the injuries of the test animals. Therefore, several in vitro tests have been developed to replace in vivo eye irritation testing. Two of these alternative methods, the MTT-Assay and the HET-CAM test (Hen’s Egg Test – Chorioallantois Membrane) were used in our comparative screening, with a set of agrochemicals to establish parallel data on in vitro and in vivo (Draize) results. The examined products were:Systhane 12 E (miclobuthanyl, 125 g/l), Clinic 480 SL (glyphosate isopropylamin salt 360 g/l), Targa Super 5 EC (quizalofop-P-ethyl 5%), TrendTM (isodecyl acetate), Silwet L-77 (polyalkylenoxid 84%, iso-propylene 16%) and Substral (66 g/l nitrogen, 30 g/l P2O5 watersoluble phosphate, 67 g/l water-soluble K2O). In these experiments, the same results were obtained in the alternative tests and in the Draize test which shows that the methods applied may be possible the replace the in vivo test as a test system in the future.

Author Biography

  • Judit Tavaszi, University of Pannonia, Georgikon Faculty, Department of Hygiene, Institute of Plant Protection H-8361 Keszthely, P. O. Box 71, Hungary

    corresponding author
    opistho@freemail.hu

References

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Published

2012-03-30

Issue

Vol. 15 No. 1 (2012)

Section

Articles

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Copyright (c) 2012 Tavaszi Judit, Kormos Éva, Pálovics Ágnes

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cikkre a Creative Commons 4.0 standard licenc alábbi típusa vonatkozik: CC-BY-NC-ND-4.0. Ennek értelmében a mű szabadon másolható, terjeszthető, bemutatható és előadható, azonban nem használható fel kereskedelmi célokra (NC), továbbá nem módosítható és nem készíthető belőle átdolgozás, származékos mű (ND). A licenc alapján a szerző vagy a jogosult által meghatározott módon fel kell tüntetni a szerző nevét és a szerzői mű címét (BY). 

How to Cite

Tavaszi, J., Kormos, Éva, & Pálovics, Ágnes. (2012). Examination of eye irritancy by using two alternative methods. GEORGIKON FOR AGRICULTURE, 15(1), 49-64. https://journal.uni-mate.hu/index.php/gfa/article/view/6740

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