Testing of pesticides for eye irritation with the HET-CAM test
Keywords:
eye irritation, pesticide, chorioallantoic membrane, HET-CAM testAbstract
There is an ongoing effort to replace the in vivo Draize test, which was generally and exclusively accepted for decades - taking into account the 3R rule and animal welfare regulations – with different alternative in vitro methods. One of these in vitro methods is the test using the chorioallantoic membrane of the hen's egg (HET-CAM). The plant protection agents included in our studies were determined using this method, and then the results were compared with the in vivo data. During the HET-CAM test, the plant protection agents were applied directly to the chorioallantoic membrane and the changes in the blood vessel system were observed. From the obtained results, it can be concluded that the results of the HET-CAM test closely approximate the in vivo data.
References
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