Germination of Erysimum pieninicum and Erysimum odoratum seeds after various storage conditions
DOI:
https://doi.org/10.56617/tl.3991Keywords:
cold treatment, germination, liquid nitrogen, seed storage, protected species, endemic species, wallflowerAbstract
Erysimum pieninicum and E. odoratum have similar morphology and habitat preference, however biogeography of the two species is contrasting. E. pieninicum is a narrow endemic, expressing a very limited distribution in SE Poland, whereas E. odoratum is rather frequent in Hungary and the surrounding countries. The aim of this paper is to investigate the germination behaviour of the two species and to highlight if the differences in the germination of the two species could be related with their distribution patterns.
Prior to germination tests, seeds of the two species were subjected to three different storage conditions:
1) room temperature (RT); 2) room temperature followed by 40 days at +4 °C before germination test (CT); 3) eight years storage in liquid nitrogen (LN) (for E. pieninicum only). After surface sterilisation of the seeds germination tests in Petri-dishes were initiated on 5th March 2009, and were terminated after 25 days.
The studied Erysimum species germinated very fast showing a germination pattern of “Erysimum wittmannii type” sensu Czarnecka and Władyka (2007). E. odoratum expressed a relatively low rate of total germination percentage (around 20%) that does not correspond previous reports. The very high germination rate of E. pieninicum (around 97% and 99%) found in RT and CT treatments, respectively, agrees with results of Czarnecka and Władyka. Cold treatment did not influence germination of the studied Erysimum species. In case of the LN treatment E. pieninicum retained a high rate of germination (81.65%).
Considering the results of the present study and also the published results of other researchers, germination behaviour of E. pieninicum does not seem to be a limiting factor for the distribution of this species. According to their seed biological characteristics (preserved viability in seed gene banks for long time and sudden germination without the need of dormancy breaking treatments) E. pieninicum and E. odoratum are promising candidates for nature conservation and habitat restoration projects.
References
Ball P. W. 2002: Erysimum L. - In: Tutin, T. G. et al. (eds.) Flora Europaea 1., pp: 325–335.
Barina Z. 2009: Brassicaceae (Cruciferae) – Keresztesvirágúak családja, pp: 168–192, in: Király G. (ed.) Új magyar füvészkönyv. Magyarország hajtásos növényei. Határozókulcsok. [New Hungarian Herbal. The Vascular Plants of Hungary. Identification key.] Aggteleki Nemzeti Park Igazgatóság, Jósvafő. (in Hungarian)
Csontos P., Simkó H. 2008: A magyar repcsény (Erysimum odoratum Ehrh.) csírázásbiológiájának vizsgálata. Tájökológiai Lapok – Journal of Landscape Eology 6(3): 247–253. (in Hungarian)
Czarnecka B., Władyka M. 2007: Ecological meaning of seed size and shape for seed persistence and germinability in some mountain plants from the collection of the Botanical Garden in Lublin. Bulletin of Botanical Gardens, Museums & Collections 16: 3–10.
Czarnecka B., Władyka M., Franszczak-Być M. 2006: Flowering phenology of some mountain plants in the collection of the Botanical Garden in Lublin. Bulletin of Botanical Gardens, Museums & Collections 15: 79–86.
Grubb P. J. 1988: The uncoupling of disturbance and recruitment, two kinds of seed bank, and persistence of plant populations at the regional and local scales. Ann. Zool. Fennici 25: 23–36.
Karlsson L. M., Milberg P. 2002: Stratification responses in the late-germinating summer annual weed Erysimum cheiranthoides. Journal of Applied BotanyAngewandte Botanik 76(5–6): 172–175.
Maciejewska-Rutkowska I., Bednorz L., Fujiki T. 2007: SEM observations of pollen grains, fruits and seeds of the Pieniny Mountains (South Poland) endemic species Erysimum pieninicum (Zapał.) Pawł. (Brassicaceae). Acta Soc. Bot. Pol. 76(2): 127–132. https://doi.org/10.5586/asbp.2007.016
Michalkova E. 2000: Chromosome numbers of Erysimum odoratum (Brassicaceae) in Slovakia. Biologia 55(4): 381–385.
Mirek Z., Piekoś-Mirkowa H., Zajac A., Zajac M. 1995: Vascular plants of Poland: a checklist. (Polish botanical studies, guidebook series; no.15). W. Szafer Institute of Botany, Polish Academy of Sciences, Krakow, 303pp. (1 computer disk – ISBN 8385444386)
Pérez-García F., González-Benito M. E., Gómez-Campo C. 2007: High viability recorded in ultra-dry seeds of 37 species of Brassicaceae after almost 40 years of storage. Seed Science and Technology 35(1): 143–153. https://doi.org/10.15258/sst.2007.35.1.13
Piekoś-Mirkowa H., Mirek Z. 2003: Endemic taxa of vascular plants in the Polish Carpathians. Acta Soc. Bot. Pol. 72(3): 235–242. https://doi.org/10.5586/asbp.2003.031
Puchalski J. T., Gawryś W. 2007: Collection of protected law and endangered plants and species protected by Bern Convention in Polish botanical gardens. Bulletin of Botanical Gardens, Museums & Collections 16: 47–184. (in Polish)
Simon T. 2000: A magyarországi edényes flóra határozója. Harasztok - virágos növények (4., átdolgozott kiadás). Nemzeti Tankönyvkiadó, Budapest, 976 pp. (in Hungarian)
Soó R. 1968: Synopsis Systematico-Geobotanica Florae Vegetationisque Hungariae III. Akadémiai Kiadó, Budapest, 506+51 pp. (in Hungarian)
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Copyright (c) 2010 Csontos Péter, Anna Rucinska, Jerzy T. Puchalski
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