Investigation of the causative agent of a virus-like symptom in grapevine
Keywords:
grapevine red blotch-associated virus, GLRaV-1, small RNA HTS, RT-PCRAbstract
Our group has been carrying on virus diagnostic surveys since 2013 using a special method: small RNA HTS. In 2018 strange symptoms, suggesting the presence of grapevine red blotchassociated virus (GRBaV), appeared at a Pinot Noir plantation. The virus was described in California in 2013 and its presence, except two descriptions (India and South-Korea) is confide to the North-American continent. To reveal the causative agent of the appeared symptoms we purified RNA from the symptomatic grapevine and made virus diagnostics by small RNA HTS. We confirmed the results using virus specific RT-PCR. Our results show that not the presence of the assumed virus cause the observed symptoms, moreover GRBaV is not present at the investigated vineyard. However, the plantation was infected with several other viruses. We think that coexistence of several different viruses together with the inhomogeneity in the soil can both contribute in the symptom development, however to clarify this question further investigations would be needed.
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Copyright (c) 2021 Dana Khrais, Demián Emese, Várallyay Éva
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