Detection of viral infections in a hungarian vineyard
Keywords:
DAS-ELISA, grapevine, virus, plant protectionAbstract
Grape (Vitis vinifera) belonging to the family of Vitaceae, is one of the most important economic fruit crops in the world. For thousands of years in Hungry, grapevine has been cultivated in the Carpathian basin, where climatic and soil conditions are suitable for grape vine production. However, it is facing adversity of virus infections that influenced negatively the performance of the cultivars at an extent, which cause premature death of the stocks and generating great yield losses. The study was conducted in a Grapevine plantation from the area of Central Transdanubia (Lesencefalu) to diagnose important viral diseases that infect Othello variety and suggest measures possible for enhancing disease control and management strategies. Othello is also a commonly cultivated variety in Hungary with the assumption of possible tolerance to important grapevine diseases. 60 samples were collected from Othello grapevine variety and analysed in the laboratory to investigate virus infection using DASELISA. Six viruses (GLRaV1, GLRaV2, GLRaV3, GLRaV6, GLRaV7 and GFkV) were found to infect this variety. Among those 60 samples, 27 samples were infected with viruses, almost half of the investigated leaf samples. The highest infection was found to be caused by GLRaV1 whilst the lowest infection was found to be associated with GLRaV3. However, 13 samples were found to be infected with more than one virus (multiple infection) whilst 14 samples were found to be infected with only one virus (single infection).
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