Determination of PPARGC1 Cys430Ser polymorphism and MHS genotype in Croatian autochthonous pig breeds

Abstract

The aim of the research was to determine PPARGC1 and MHS genetic status of two Croatian autochthonous pig breeds. The research was carried out on 20 Black Slavonian pigs and 20 Turopolje pigs. Genomic DNA was extracted from the blood. PCR was performed and PCR products genotyped by Restriction Fragment Length Polymorphism (RFLP). Allele frequency for PPRGC1 gene was determined by the Hardy-Weinberg equilibrium principle. Research results showed that A allele frequency in Black Slavonian pig was 44.44%, and of the T allele 55.56%. Homozygous genotypes (Ser/Ser) were dominant in Turopolje pig breeds (A=100.00%). Determined MHS status proved both pig breeds to be completely MHS resistant. Our results point out the necessity to further investigate genetic status of both pig breeds in order to improve constitution and quality traits of muscle tissue in pure pig breeds.