Adiponectin gene expression in muscle and fat tissues of Mangalica, Mangalica×Duroc, and Hungarian LargeWhite pigs
DOI:
https://doi.org/10.17205/SZIE.AWETH.2015.2.193Keywords:
adiponectin, Mangalica, Hungarian Large White, gene expressionAbstract
The adipocyte-secreted protein hormone adiponectin plays roles in the regulation of gluconeogenesis and the oxidation of fatty acids. Muscle and backfat tissue samples were collected from purebred and Duroc crossbred Blond Mangalica and Hungarian Large White (HLW) pigs at an abattoir in order to determine the expression level of adiponectin (ADIPOQ) gene in the different breeds and tissues. The RNA isolation was carried out by means of the TRIzol protocol, and the expression was normalised to β-actin reference gene and was analysed by the 2-ΔΔCt method. In each of the three groups, the ADIPOQ expression was significantly higher (P<0.05) in backfat tissues compared to that in muscle samples. There was no significant (P<0.05) difference between the ADIPOQ expression in backfat tissues of purebred and crossbred Mangalica and HLW; however, lowest expression was detected in HLW samples. Muscle ADIPOQ expression was significantly (P<0.05) higher in purebred and crossbred Mangalica than in HLW. Variations in muscle ADIPOQ expression can be explained by, and contribute to differences in intramuscular fat accumulation between Mangalica and HLW.
References
Chomczynski, P., Sacchi, N. (1987): Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1) 156–159. https://doi.org/10.1016/0003-2697(87)90021-2
Cirera, S., Jensen, M. S., Elbrond, V. S., Moesgaard, S. G., Christoffersen, B. O., Kadarmideen, H. N., Skovgaard, K., Bruun, C. V., Karlskov-Mortensen, P., Jorgensen, C. B., Fredholm, M. (2013): Expression studies of six human obesity-related genes in seven tissues from divergent pig breeds. Animal Genetics, 45(1) 59–66. https://doi.org/10.1111/age.12082
Daniele, A., Cammarata, R., Masullo, M., Nerone, G., Finamore, F., D’Andrea, M., Pilla, F., Oriani, G. (2008): Analysis of adiponectin gene and comparison of its expression in two different pig breeds. Obesity, 16(8) 1869–1874. https://doi.org/10.1038/oby.2008.275
Ding, S. T., Liu, B. H., Ko, Y. H. (2004): Cloning and expression of porcine adiponectin and adiponectin receptor 1 and 2 genes in pigs. Journal of Animal Science, 82(11) 3162–3174. https://doi.org/10.2527/2004.82113162x
Gable, D. R., Hurel, S. J., Humphries, S. E. (2006): Adiponectin and its gene variants as risk factors for insulin resistance, the metabolic syndrome and cardiovascular disease. Atherosclerosis, 188(2) 231–244. https://doi.org/10.1016/j.atherosclerosis.2006.02.010
Hu, E., Liang, P., Spiegelman, B. M. (1996): AdipoQ is a novel adipose-specific gene dysregulated in obesity. Journal of Biological Chemistry, 271(18) 10697–10703. https://doi.org/10.1074/jbc.271.18.10697
Luo, H. F., Wei, H. K., Huang, F. R., Zhou, Z., Jiang, S. W., Peng, J. (2009): The effect of linseed on intramuscular fat content and adipogenesis related genes in skeletal muscle of pigs. Lipids, 44(11) 999–1010. https://doi.org/10.1007/s11745-009-3346-y
Maeda, K., Okubo, K., Shimomura, I., Funahashi, T., Matsuzawa, Y., Matsubara, K. (1996): cDNA cloning and expression of a novel adipose specific collagen-like factor, apMI (AdiPose Most abundant Gene transcript 1). Biochemical and Biophysical Research Communications, 221(2) 286–289. https://doi.org/10.1006/bbrc.1996.0587
Wang, Y., Lam, K. S. L., Xu, A. (2006): Adiponectin as a therapeutic target for obesity-related metabolic and cardiovascular disorders. Drug Development Research, 67(8) 677–686. https://doi.org/10.1002/ddr.20141
Downloads
Published
Issue
Section
License
Copyright (c) 2015 Tempfli Károly, Kovács Bálint, Posgay Miklós, Simon Zoltán, Bali Papp Ágnes
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
