Investigation of effects caused by water disinfection byproduct (3-Amino-9 ethylcarbazole) on zebrafish using toxicological tests and gene expression analysis

Authors

  • Erna Balogh SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Roberta Berta SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Gyöngyi Gazsi SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Edina Garai SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Márta Reining SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Béla Urbányi SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Katalin Bakos SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.
  • Zsolt Csenki SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.

DOI:

https://doi.org/10.17205/SZIE.AWETH.2019.1.001

Keywords:

zebrafish, toxikology, water disinfection byproduct

Abstract

The aim of this study was the investigation of toxic effects of 3-Amino-9 ethylcarbazole (3A9EC) water disinfection byproduct. Tests were carried out according to the modified OECD (Organisation for Economic Cooperation and Development) guideline (No. 236) in order to detect the acute and sub-chronic toxic effects of 3A9EC.

At the end of exposure, LC50 and LC10 values for embryo mortality were determined.

Next, a subchronic assay of 33 days was conducted to study the lethal and sub-lethal effects of 3A9EC on the early life stage of zebrafish. On the basis of acute test results, embryos were exposed to two test concentrations below the 72h LC10. At the end of the test, only those individuals survived which were treated with the lower concentrations of 3A9EC. Treated embryos showed developmental malfunctions, differences in size and depigmentation compared to the control group.

To observe vascular and nervous system effects, experiments were conducted on three transgenic zebrafish lines. Impairments were only detected in 72 hours post fertilization (hpf) larvae.

However results provide complex information on the toxic effects of 3A9EC, it seems that the substance could be potentially harmful to living organisms including humans, so further experiments are needed.

Author Biography

  • Erna Balogh, SZIE Mezőgazdaság- és Környezettudományi Kar Akvakultúra és Környezetbiztonsági Intézet, 2100 Gödöllő, Páter Károly utca 1.

    corresponding author
    balogh.erna@mkk.szie.hu

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Published

2019-04-30

Issue

Vol. 15 No. 1 (2019): AWETH

Section

Cikk szövege

License

Copyright (c) 2019 Balogh Erna, Berta Roberta, Gazsi Gyöngyi, Garai Edina, Reining Márta, Urbányi Béla, Bakos Katalin, Csenki Zsolt

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Investigation of effects caused by water disinfection byproduct (3-Amino-9 ethylcarbazole) on zebrafish using toxicological tests and gene expression analysis. (2019). Animal Welfare, Ethology and Housing Systems (AWETH), 15(1), 1-8. https://doi.org/10.17205/SZIE.AWETH.2019.1.001

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