Magyarországon megjelenő új szőlő vírusok azonosítása kis RNS-ek szekvenálásán alapuló metagenomikai módszerekkel
Keywords:
grapevine, virus, diagnostics, smallRNA-NGSAbstract
Among several grapevine infecting pathogens there are more than 60 viruses and viroids. Lifespan of grapevine plantations are greatly influenced by their possible infection with viruses and viroids. In our work instead of traditional virus diagnostics methods we used a basically new strategy to detect virus infection in grapevine: next generation sequencing of virus derived small RNAs. We used this technique to determine the presence of these pathogens in grapevine plantations from distant part of the country. During our survey we detected widespread distribution of two viruses, never described in our country before: Grapevine Pinot gris virus and Grapevine Syrah virus1. Their presence was validated by RT-PCR and traditional Sanger sequencing.
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Copyright (c) 2016 Czotter Nikoletta, Molnár János, Deák Tamás, Tusnády E. Gábor, Kocsis László, Burgyán József, Várallyay Éva
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