Testing of pesticides for eye irritation with the HET-CAM test

Authors

  • Svetlana Kazarova Hungarian University of Agricultural and Life Sciences, Institute of Plant Protection, Department of Plant Protection, e-mail: skazarova1997@mail.ru (corresponding author) https://orcid.org/0000-0002-4594-5118
  • Esther Ijeoma Idogwu Hungarian University of Agricultural and Life Sciences, Institute of Plant Protection, Department of Plant Protection, e-mail: Idogwu.Esther.Ijeoma@phd.uni-mate.hu
  • Éva Kormos Hungarian University of Agricultural and Life Sciences, Institute of Plant Protection, Department of Plant Protection, e-mail: Kormos.Eva@uni-mate.hu https://orcid.org/0000-0003-2160-3153

Keywords:

eye irritation, pesticide, chorioallantoic membrane, HET-CAM test

Abstract

There is an ongoing effort to replace the in vivo Draize test, which was generally and exclusively accepted for decades - taking into account the 3R rule and animal welfare regulations – with different alternative in vitro methods. One of these in vitro methods is the test using the chorioallantoic membrane of the hen's egg (HET-CAM). The plant protection agents included in our studies were determined using this method, and then the results were compared with the in vivo data. During the HET-CAM test, the plant protection agents were applied directly to the chorioallantoic membrane and the changes in the blood vessel system were observed. From the obtained results, it can be concluded that the results of the HET-CAM test closely approximate the in vivo data.

References

Bagley, D. M., Bruner, L. H. and De Silva, O. 1992. An evaluation of five potential alternatives in vitro to the rabbit eye irritation test in vivo. Toxicology in Vitro 6. 275-284.

Bagley, D. M., Waters, D. and Kong, B. M. 1994. Development of a 10-day chorioallantoic membrane vascular assay as an alternative to the Draize rabbit eye irritation test. Food and Chemical Toxicology 33. 1155-1160.

Draize, J., Woodard, G. and Calvery, H. 1944. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. Journal of Pharmacology & Experimental Therapeutics 82. 377-390.

Invittox Protocol Number 47. 1990. HET-CAM Test, ISSN 0960–219

Jírová, D., Kejlová, K., Janoušek, S., Bendová, H., Malý, M., Kolářová, H. and Dvořáková, M. 2014. Eye irritation hazard of chemicals and formulations assessed by methods in vitro. Neuroendocrinology Letters 35. 133-140.

Kormos, E., Lehel, J. and Haszon, K. 2011. Comparative study with a set of pesticides to establish eye irritancy with HET-CAM and Draize test. Georgikon for Agriculture 14(1) 39-51.

Leighton, J., Nassauer, J. and Tchao, R. 1985. The chick embryo in toxicology: an alternative to the rabbit eye. Food and Chemical Toxicology 23(2). 293-298.

Russell, W. M. S. and Burch, R. L. 1959. The principles of humane experimental technique, chapter 4. Methouen, London

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Published

2023-11-30

Issue

Vol. 27 No. 1 (2023): Supplement: XXXII. Keszthelyi Növényvédelmi Fórum

Section

Articles

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Copyright (c) 2023 Svetlana Kazarova, Esther Ijeoma Idogwu, Éva Kormos

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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