Heat resistance of Clostridium perfringens spores

Abstract

The purpose of our researches is to find out the minimal heat-dose, which is enough for canning the seafowl liver conserves. It is crucial that the temperature, used during the heat treating cannot exceed 100 °C. Clostridium perfringens NCAIM-B-01417 tribe was developed in a certain broth, then the developed spore suspension was used for the heat treatment in 80 °C, 85 °C, 90 °C, 95 °C. During the heat treatment researches we kept the spore suspension on 80 and 85 °C for 45 minutes, on 90 °C for 15 minutes, on 95 °C for 6 minutes. After considering the parameters, it was declared that in for the Clostridium perfringens NCAIM-B-01417 tribe on 85 °C temperature 29 minutes, on 95 °C 5 minutes were needed for decreasing the number of spores with log2. Consequently, it is possible to use lower temperature for the safe elimination of spores.