Identification and expression of pollen allergen transcripts in different organs of the common ragweed (Ambrosia artemisiifolia l.)
Keywords:
Ambrosia artemisiifolia, common ragweed, pollen allergen, Amb a, transcript, gene expressionAbstract
An increasing number of individuals are affected in the temperate climate zone by allergy caused by the pollen of the common ragweed (Ambrosia artemisiifolia L.). For 11 allergen families of ragweed the polypeptide sequences are available, and for most the nucleotide sequence is also known. However, little is known about the expression of these allergen genes. In the present study transcriptomes of the male and female flowers, as well as of leaves were produced and transcripts with high similarity or identity to the allergen genes were identified. All the analyzed 11 allergen genes could be detected in the male flowers and with the exception of Amb a 6 also in leaves. Amb a 1, Amb a 3, Amb a 8 and Amb a 12 were expressed also in female flowers. The strongest expression was found for Amb a 5 and then for Amb a 3. Our results indicate that expression of ragweed pollen allergens is not restricted to the pollen, but they function also in other organs of the plant. The results presented here contribute to our understanding on the function and role of the genes of pollen allergens in common ragweed.
References
Alvarez-Buylla E.R., Benítez M., Corvera-Poiré A., Cador Á.C., de Folter S., de Buen A.G., Garay-Arroyo A., García-Ponce B., JaimesMiranda F., Pérez-Ruiz R.V., PiñeyroNelson A., Sánchez-Corralesa Y.E. Flower Development. In: The Arabidopsis Book. The American Society of Plant Biologists; Rockville, MD.: 2010 (8) http://www.aspb.org/publications/arabidopsis/. https://doi.org/10.1199/tab.0127
Augustin, S., Stock, M., Cromwell, O., Nandy, A., Reese, G. 2012. Proteomic and immunological characterization of ragweed allergens. World Allergy Organ Journal. 5 (2) S23–S24. https://doi.org/10.1097/01.WOX.0000411763.28401.42
Augustin, S., Wald, M., Asero, R., Reese, G., Klysner, S., Nandy, A. 2013. Assessment of Amb a 1 isoallergens as basis for development of a recombinant ragweed immunotherapeutic vaccine. Allergy. 68 (97) 111.
Bouley, J., Groeme, R., Le Mignon, M., Jain, K., Chabre, H., Bordas-Le Floch, V., Couret, M., Bussières, L., Lautrette, A., Naveau, M., Baron-Bodo, V., Lombardi, V., Mascarell, L., Batard, T., Nony, E.,Moingeon, P. 2015. Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed. Journal of Allergy and Clinical Immunology. 136 (4) 1055–1064. https://doi.org/10.1016/j.jaci.2015.03.001
Bordas-Le Floch, V., Groeme, R.,Chabre, H., Baron-Bodo, V., Nony E., Mascarell, L., Moingeon, P. 2015a. New insights into ragweed pollen allergens. Current Allergy and Asthma Reports. 15 (11) 63. https://doi.org/10.1007/s11882-015-0565-6
Bordas-Le Floch, V., Le Mignon, M., Bouley, J., Groeme, R., Jain, K., Baron-Bodo, V., Nony, E., Mascarell, E., Moingeon, E. 2015b. Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches. PLoS ONE 10 (8) 1–18. https://doi.org/10.1371/journal.pone.0136258
Chen, M., Xu, J., Devis, D., Shi, J., Ren, K., Searle, I., Zhang, D. 2016. Origin and Functional Prediction of Pollen Allergens in Plants. Plant Physiology. 172 (1) 341–357. https://doi.org/10.1104/pp.16.00625
Ghosh, B., Perry, M. P., Rafnar, T., Marsh, D. G. 1993. Cloning and expression of immunologically active recombinant Amb a V allergen of short ragweed (Ambrosia artemisiifolia) pollen. Journal of Immunology. 150 (12) 5391–5392. https://doi.org/10.4049/jimmunol.150.12.5391
Hiller, K. M., Lubahn, B. C., Klapper, D. G. 1998. Cloning and expression of ragweed allergen Amb a 6. Scandinavian Journal of Immunology. 48 (1) 26–36. https://doi.org/10.1046/j.1365-3083.1998.00355.x
Kazinczi, G., Béres, I., Novák, R., Bíro, K., Pathy, Z. 2008. Common ragweed (Ambrosia artemisiifolia): A review with special regards to the result in Hungary I. Taxonomy, origin and distribution, morphology, life cycle and reproduction strategy. Herbologia. 9. 55–91.
Klapper, D. G., Goodfriend, L., Capra, J. D. 1980. Aminoacid sequence of ragweed allergen Ra3. Biochemistry. 19 (25) 5729–5734. https://doi.org/10.1021/bi00566a010
Léonard, R., Wopfner, N., Pabst, M., Stadlmann, J., Petersen, B. O., Duus, J., Himly, M., Radauer, C., Gadermaier, G., Razzazi-Fazeli, E., Ferreira, F., Altmann, F. 2010. A new allergen from ragweed (Ambrosia artemisiifolia) with homology to Art v 1 from mugwort. The Journal of Biological Chemistry. 285 (35) 27192–27200. https://doi.org/10.1074/jbc.M110.127118
Marín-Rodriguez, M. C., Orchard, J., Seymour, G. B. 2002. Pectate lyases, cell wall degradation and fruit softening. Journal of Experimental Botany. 53 (377) 2115–2119. https://doi.org/10.1093/jxb/erf089
Metzler, W. J., Valentine, K., Roebber, M., Marsh, D.G., Mueller, L. 1992. Proton resonance assignments and three-dimensional solution structure of the ragweed allergen Amb a V by nuclear magnetic resonance spectroscopy. Biochemistry. 31 (37) 8697–705. https://doi.org/10.1021/bi00152a003
Nandy, A., Augustin, S., Mitulski, L., Cromwell, O. 2011. Isoallergen analysis of pectate lyases (Amb a 1 and Amb a 2) from commercial short ragweed pollen. Journal of Allergy and Clinical Immunology. 127 (2) AB168. https://doi.org/10.1016/j.jaci.2010.12.668
Novák, R., Dancza, I., Szentey, L., Karamán J. (2009). Magyarország szántóföldjeinek gyomnövényzete. (in Hungarian). Ötödik Országos Szántóföldi Gyomfelvételezés. FVM, Budapest.
Rafnar, T., Griffith, I. J., Kuo, M., Bond, J. F., Rogers, B. L., Klapper, D. G. 1991.Cloning of Amb a I (Antigen E), the major allergen family of short ragweed pollen. The Journal of Biological Chemistry. 266 (2) 1229–1236. https://doi.org/10.1016/S0021-9258(17)35305-X
Roebber, M., Hussain, R., Klapper, D. G., Marsh, D. G., 1983. Isolation and properties of a new short ragweed pollen allergen, Ra6. Journal of Immunology. 131 (2) 706–711. https://doi.org/10.4049/jimmunol.131.2.706
Roebber, M., Marsh, D. G. 1991. Isolation and characterization of allergen Amb a VII from short ragweed pollen. Journal of Allergy and Clinical Immunology. 87 (1) 324. https://doi.org/10.1016/0091-6749(91)92021-R
Rogers, B. L., Pollock, J., Klapper, D.G., Griffith, I. J. 1993. Sequence of the proteinase-inhibitor cystatin homologue from the pollen of Ambrosia artemisiifolia (short ragweed). Gene. 133 (2) 219–221. https://doi.org/10.1016/0378-1119(93)90641-F
Smyth, D. R., Bowman J. L., Meyerowitz E. M. 1990. Early flower development in Arabidopsis. Plant Cell. 2 (8) 755–767. https://doi.org/10.1105/tpc.2.8.755
Taller, J., Decsi, K., Farkas, E., Nagy, E., Mátyás, K. K., Kolics, B., Kutasy, B., Virág, E. 2016. De novo transcriptome sequencing based identification of Amb a 3-like pollen allergen in common ragweed (Ambrosia artemisiifolia). Journal of Botanical Science 5 (2) 36–40.
Taniguchi, Y., Ono, A., Sawatani, M., Nanba, M., Kohno, K., Usui, M., Kurimoto, M., Matuhasi, T. 1995. Cry j I, a major allergen of Japanese cedar pollen, has a pectate lyase enzyme activity. Allergy 50 (1) 90–93. https://doi.org/10.1111/j.1398-9995.1995.tb02489.x
Virág, E. and Hegedűs, G. 2018. Digitális gén expressziós elemzések szoftveres támogatása. 48. Membrán transzport konferencia, Sümeg, Május 15–18. 2018. (poszter) (in Hungarian)
Virág E., Hegedűs G., Barta E., Nagy E., Mátyás K., Kolics B., Taller J. 2016. Illumina Sequencing of Common (Short) Ragweed (Ambrosia artemisiifolia L.) Reproductive Organs and Leaves. Frontiers in Plant Science. 7. 1506 https://doi.org/10.3389/fpls.2016.01506
Wopfner, N., Gruber, P., Wallner, M., Briza, P., Ebner, C., Mari, A., Richter, K., Vogel, L., Ferreira, F. 2008. Molecular and immunological characterization of novel weed pollen pan-allergens. Allergy. 63 (7) 872–881. https://doi.org/10.1111/j.1398-9995.2008.01635.x
Downloads
Published
Issue
Section
License
Copyright (c) 2019 Farkas Eszter, Farkas Zoltán, Hegedűs Géza, Kutassy Barbara, Kolics Balázs, Mátyás Kinga Klára, Parrag Tamás, Solti Izabella, Virág Eszter, Taller János
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Cikkre a Creative Commons 4.0 standard licenc alábbi típusa vonatkozik: CC-BY-NC-ND-4.0. Ennek értelmében a mű szabadon másolható, terjeszthető, bemutatható és előadható, azonban nem használható fel kereskedelmi célokra (NC), továbbá nem módosítható és nem készíthető belőle átdolgozás, származékos mű (ND). A licenc alapján a szerző vagy a jogosult által meghatározott módon fel kell tüntetni a szerző nevét és a szerzői mű címét (BY).
