Study of pigment production of Yarrowia strains
Keywords:
Yarrowia lipolytica, pigment production, nutrient medium optimisationAbstract
In addition to many metabolic products, the yeast Y. lipolytica is also capable of producing dyes, which, due to their natural origin, can be used in various industries as an alternative to synthetic pigments. In the course of our research, in addition to Y. lipolytica, isolates belonging to other Yarrowia species were examined in terms of pigment production, and nutrient optimization experiments were carried out to increase pigment production. Y. lipolytica t 6/3 proved to be the best pigment-producing strain, and the most effective medium contained additional amino acids (glycine, L-glutamine, L-asparagine) in addition to tyrosine. During the examination of the effect of pH, we found that moving away from neutral, whether in an acidic or alkaline range, the level of pigment production decreased. In the pH=7 buffered medium, similar results were obtained to those prepared with distilled water, and pigment production reached its maximum on the seventh day of fermentation. Manganese significantly affects the production of the brown pigment, with the application of 5 mM, the pigment production increased almost fivefold.
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Copyright (c) 2022 Sipiczki Gizella, Eszterbauer Edina , Nagy Edina Szandra, Kiss Zsuzsanna, Bujna Erika
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