Cardiac responses to transrectal examination of lactating and non-lactating dairy cows
Keywords:
heart rate, heart rate variability, palpation per rectum, pain, dairy cowAbstract
In this study we evaluated heart rate (HR) and parasympathetic parameters of heart rate variability (HRV) to monitor cardiac stress responses to palpation per rectum in lactating (n = 11) and non-lactating (n = 12) dairy cows. HR and HRV were measured from 40 min before the examination until 120 min after it was done. HR, the root mean square of successive differences (RMSSD) and the high frequency component (HF) of HRV were analyzed in equal length of 5 min data segments. To compare cardiac responses to palpation per rectum between groups, changes in HR and HRV parameters were calculated as area under the curve (AUC). Although an immediate increase was found in HR during the examination in both lactating (+21.4 ± 2.4 beats/min) and non-lactating animals (+20.6 ± 2.3 beats/min), no differences were detected between groups using by the AUC method. The rise of HR in both groups along with a parallel decline of RMSSD (lactating cows: –5.2 ± 0.4 ms, non-lactating cows: –5.1 ± 0.4 ms) and HF (lactating cows: –10.1 ± 0.8 nu, non-lactating cows: –16.9 ± 1.2 nu) during transrectal palpation reflected to an increase in the sympathetic, and a decrease in the vagal tone. The amplitude and the maximum RMSSD and HF values were higher in non-lactating cows than in lactating animals suggesting a higher short-term cardiac responsiveness of non-lactating ones. However, the magnitude and the duration of the stress response were greater in lactating cows, as indicated by the analysis of AUC. Cows’ response to stress experienced during and after palpation per rectum was more prominent in vagal indices of HRV than in HR. Based on our results, the impact of transrectal examination on the cows’ cardiac stress responses may have an impact on animal welfare on dairy farms. Further research is required to find out whether the differences due to lactation are physiological or management-related.
References
Akers, R. M., Denbow, D. M. (2013): Peripherial and autonomic nervous system, pp. 265–296. In: Akers, R. M., Denbow, D. M. (eds.): Anatomy and physiology of domestic animals, John Wiley & Sons Inc., Iowa, USA, pp. 633.
Borell von, E., Langbein, J., Després, G., Hansen, S., Leterrier, C., Marchant-Forde, J., Marchant-Forde, R., Minero, M., Mohr, E., Prunier, A., Valance, D., Veissier, I. (2007): Heart rate variability as a measure of autonomic regulation of cardiac activity for assessing stress and welfare in farm animals: a review. Physiology and Behavior, 92(3) 293–316. https://doi.org/10.1016/j.physbeh.2007.01.007
Cingi, C. C., Baser, D. F., Karafakioglu, Y. S., Fidan, A. F. (2012): Stress Response in Dairy Cows Related to Rectal Examination. Acta Scientiae Veterinariae, 40(3) 1053.
Hagen, K., Lexer, D., Palme, R., Troxler, J., Waiblinger, S. (2004): Milking of Brown Swiss and Austrian Simmental cows in a herringbone parlour or an automatic milking unit. Applied Animal Behaviour Science, 88(3–4) 209–225. https://doi.org/10.1016/j.applanim.2004.03.005
Hainsworth, R. (1995): The control and physiological importance of heart rate. In Heart rate variability, pp. 3–19. In: Malik, M., Camm, A.J. (eds.): Heart rate variability. Futura Publishing, Armonk, New York, USA, pp. 211.
Koenig J., Jarczok, M. N., Ellis, R. J., Hillecke, T. K., Thayer, J. F. (2013): Heart rate variability and experimentally induced pain in healthy adults: A systematic review. European Journal of Pain, 18(3) 301–314. https://doi.org/10.1002/j.1532-2149.2013.00379.x
Lay Jr, D. C., Friend, T. H., Randel, R. D., Jenkins, O. C., Neuendorff, D. A., Kapp, G. M., Bushong, D. M. (1996): Adrenocorticotropic hormone dose response and some physiological effects of transportation on pregnant Brahman cattle. Journal of Animal Science, 74(8) 1806–1811. https://doi.org/10.2527/1996.7481806x
LeBlanc, S. J., Duffield, T. F., Leslie, K. E., Bateman, K. G., Keefe, G. P., Walton, J. S., Johnson, W. H. (2002): Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal of Dairy Science, 85(9) 2223–2236. https://doi.org/10.3168/jds.S0022-0302(02)74302-6
Ledowski, T., Reimer, M., Chavez, V., Kapoor, V., Wenk, M. (2012): Effects of acute postoperative pain on catecholamine plasma levels, hemodynamic parameters, and cardiac autonomic control. Pain, 153(4) 759–764. https://doi.org/10.1016/j.pain.2011.11.002
Martí, O., García, A., Vellès, A., Harbuz, M. S., Armario, A. (2001): Evidence that a single exposure to aversive stimuli triggers long-lasting effects in the hypothalamus-pituitary-adrenal axis that consolidate with time. European Journal of Neuroscience, 13(1) 129–136. https://doi.org/10.1111/j.1460-9568.2001.01355.x
Mohr, E., Langbein, J., Nürnberg, G. (2002): Heart rate variability: A noninvasive approach to measure stress in calves and cows. Physiology and Behavior, 75(1–2) 251–259. https://doi.org/10.1016/S0031-9384(01)00651-5
Nakao, T., Sato, T., Moriyoshi, M., Kawata, K. (1994): Plasma cortisol response in dairy cows to vaginoscopy, genital palpation per rectum and artificial insemination. Journal of Veterinary Medicine, 41(1) 16–21. https://doi.org/10.1111/j.1439-0442.1994.tb00060.x
Ness, T. J., Gebhart G. F. (1990): Visceral pain: a review of experimental studies. Pain, 41(2) 167–234. https://doi.org/10.1016/0304-3959(90)90021-5
Petyim, S., Bage, R., Madej, A., Larsson, B. (2007): Ovum pick-up in dairy heifers: does it affect animal well-being? Reproduction of Domestic Animals, 42(6) 623–632. https://doi.org/10.1111/j.1439-0531.2006.00833.x
Pilz, M., Fischer-Tenhagen, C., Thiele, G., Tinge, H., Lotz, F., Heuwieser, W. (2012): Behavioural reactions before and during vaginal examination in dairy cows. Applied Animal Behaviour Science, 138(1–2) 18–27. https://doi.org/10.1016/j.applanim.2012.01.011
Porges, S. W. (2003): The polyvagal theory: phylogenetic contributions to social behavior. Physiology and Behavior, 79(3) 503–513. https://doi.org/10.1016/S0031-9384(03)00156-2
Rietmann, T. R., Stauffacher, M., Bernasconi, P., Auer, J. A., Weishaupt, M. A. (2004): The association between heart rate, heart rate variability, endocrine and behavioural pain measures in horses suffering from laminitis. Journal of Veterinary Medicine, 51(5) 218–225. https://doi.org/10.1111/j.1439-0442.2004.00627.x
Romano, J. E., Thompson, J. A., Kraemer, D. C., Westhusin, M. E., Forrest, D. W., Tomaszweski, M. A. (2007): Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle. Theriogenology, 67(3) 486–493. https://doi.org/10.1016/j.theriogenology.2006.08.011
Sayers, B. M. (1973): Analysis of heart rate variability. Ergonomics, 16(1) 17–32. https://doi.org/10.1080/00140137308924479
Stewart, M., Verkerk, G. A., Stafford, K. J., Schaefer, A. L., Webster, J. R. (2010): Noninvasive assessment of autonomic activity for evaluation of pain in calves, using surgical castration as a model. Journal of Dairy Science, 93(8) 3602–3609. https://doi.org/10.3168/jds.2010-3114
Stubsjøen, S. M., Flø, A. S. Moe, R. O. Janczak, A. M. Skjerve, E. Valle, P. S., Zanella, A. J. (2009): Exploring non-invasive methods to assess pain in sheep. Physiology and Behavior, 98(5) 640–648. https://doi.org/10.1016/j.physbeh.2009.09.019
Terkelsen, A. J., Andersen, O. K., Mølgaard, H., Hansen, J., Jensen, T. S. (2004): Mental stress inhibits pain perception and heart rate variability but not a nociceptive withdrawal reflex. Acta Physiologica Scandinavica, 180(4) 405–414. https://doi.org/10.1111/j.1365-201X.2004.01263.x
Waiblinger, S., Menke, C., Korf, J., Bucher, A. (2004): Previous handling and gentle interactions affect behaviour and heart rate of dairy cows during a veterinary procedure. Applied Animal Behaviour Science, 85(1–2) 31–42. https://doi.org/10.1016/j.applanim.2003.07.002
Youngquist, R. S. (1997): Pregnancy diagnosis, pp. 295–303. In: Youngquist, R. S. (ed.): Current Therapy in Large Animal Theriogenology. WB Saunders Company, St. Louis, USA, pp. 898.
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Copyright (c) 2014 Kézér Fruzsina Luca, Kovács Levente, Jurkovich Viktor, Tőzsér János
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