Determination of PPARGC1 Cys430Ser polymorphism and MHS genotype in Croatian autochthonous pig breeds

Szerzők

  • Vladimir Margeta Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia
  • Polonca Frajman University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, 1230 Domžale, Slovenia
  • Gordana Kralik Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia
  • Peter Dovč University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, 1230 Domžale, Slovenia

Kulcsszavak:

PPARGC1, MHS, Black Slavonian pig, Turopolje pig, allele frequency

Absztrakt

The aim of the research was to determine PPARGC1 and MHS genetic status of two Croatian autochthonous pig breeds. The research was carried out on 20 Black Slavonian pigs and 20 Turopolje pigs. Genomic DNA was extracted from the blood. PCR was performed and PCR products genotyped by Restriction Fragment Length Polymorphism (RFLP). Allele frequency for PPRGC1 gene was determined by the Hardy-Weinberg equilibrium principle. Research results showed that A allele frequency in Black Slavonian pig was 44.44%, and of the T allele 55.56%. Homozygous genotypes (Ser/Ser) were dominant in Turopolje pig breeds (A = 100.00%). Determined MHS status proved both pig breeds to be completely MHS resistant. Our results point out the necessity to further investigate genetic status of both pig breeds in order to improve constitution and quality traits of muscle tissue in pure pig breeds.

Információk a szerzőről

  • Vladimir Margeta, Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia

    levelezőszerző
    vmargeta@pfos.hr

Hivatkozások

Aalhus, J. L., Jones, W. M., Robertson, W. M., Tong, A. K. W., Sather, A. P. (1991). Growth characteristics and carcass composition of pigs with known genotypes for stress susceptibility over a weight range of 70 to 120 kg. Anim. Prod., 52(2), 347. https://doi.org/10.1017/S0003356100012873

Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K. (2000). Current Protocols in Molecular Biology. Vol. I, section 2. and 3., John Wiley and Sons, Inc., New York. https://doi.org/10.1002/0471142727

Denborough, M. (1988). Malignant Hyperthermia. Lancet, 352(9134), 1131–1136. https://doi.org/10.1016/S0140-6736(98)03078-5

Đikić, M., Jurić, I., Rupić, V., Gomerčić, H., Đikić, D. (2002). Turopoljska svinja, sastav trupa masnih kiselina tkiva. 7th World Congress on Genetics Applied to Livestock Production, Montpellier, August 19–23, Sess., 25, No. Comm. 25–32.

Fuji, J., Otsu, K., Zorzato, F. (1991). Identification of a mutation in the porcine ryanodine receptor associated with malignant hyperthermia. Science. 253(5018), 448–451. https://doi.org/10.1126/science.1862346

Houde, A., Godbout, D., Gariepy, C. (2001). Major Genes and Meat Quality. Advances in Pork Production. 12. 139–142.

Petricevic, A., Kralik, G., Petrovic, D. (1988). Participation and Quality of some Tissues in Pig Carcasses of Different Production Types. 34th International Congress of Meat Science and Technology. Brisbane, Australia, 68.

Kralik, G., Petricevic, A., Levakovic, F. (1988). Slaughter Value of Pigs of Different Production Types. 34th International Congress of Meat Science and Technology. Brisbane, Australia, 88.

Kunej, T., Wu, H. L., Berlic, T. M., Michal, J. J., Jiang, Z., Dovc, P. (2005). Frequency distribution of a Cys430Ser polymorphism in peroxime proliferator-activated receptor-gamma coactivator-1 (PPARGC1) gene sequence in Chinese and Western pig breeds. J. Anim. Breed. Genet., 122(1), 7–11. https://doi.org/10.1111/j.1439-0388.2004.00498.x

Lowel, B. B., Spiegelman, B. M. (2000). Towards molecular understanding of adaptive thermogenesis. Nature. 404. 562–660. https://doi.org/10.1038/35007527

Pommier, S. A., Houde, A., Rousseau, F., Savoie, Y. (1992). The effect of the malignant hyperthermia genotype as determined by a restriction endonuclease assay on carcass characteristics of commercial crossbred pigs. Can. J. Anim. Sci., 72(4), 973–976. https://doi.org/10.4141/cjas92-111

Puigserver, P., Spiegelman, B. M. (2003). Peroxisome Proliferator-Activated Receptor- (gamma) Coactivator 1(alpha) PGC-1(alpha): Transcriptional Coactivator and Metabolic Regulator. Endocr. Rev., 24(1), 78–90. https://doi.org/10.1210/er.2002-0012

Rosner, F., Von Langerken, G., Maak, S. (2003). The value of pig breeding herds in Germany and progress in improvement of meatiness and pork quality. Animal Science Papers and Reports. 21(1), 153–161.

Rűbensam, J. M. (2000). Post mortem changes and pork quality. 1st International Virtual Conference of Pork Quality.

Letöltések

Megjelent

2006-07-15

Folyóirat szám

Évf. 10 szám 2 (2006): Acta Agraria Kaposváriensis

Rovat

Poster Section

License

Copyright (c) 2006 Vladimir Margeta, Polonca Frajman, Gordana Kralik, Peter Dovč

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hogyan kell idézni

Margeta, V., Frajman, P., Kralik, G., & Dovč, P. (2006). Determination of PPARGC1 Cys430Ser polymorphism and MHS genotype in Croatian autochthonous pig breeds. Acta Agraria Kaposváriensis, 10(2), 333-339. https://journal.uni-mate.hu/index.php/aak/article/view/1817

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