In silico identification of putative barley phytochrome interacting factors (PIFs)
Keywords:
barley, in silico analysis, phylogenetic tree, bHLH protein family, PIFAbstract
Phytochrome Interacting Factors (PIFs) are plant transcription factors; members of the basic helix-loop-helix (bHLH) protein family. PIFs interact with the light sensitive phytochrome photoreceptors, thus they play pivotal role in light signaling, influencing a vast number of physiological processes. In spite of their importance, only a few studies focus on the identification of PIFs in different plant species until now. In this in silico study, we identified barley (Hordeum vulgare L.) bHLH proteins, and divided them into subfamilies by phylogenetic analysis. A total of 9 barley bHLH sequences were classified as VII (a+b) subfamily members. Since this group contains also those sequences, which were applied as reference PIF proteins (isolated from other plant species), we consider these 9 proteins as putative barley PIFs. These results provide a useful dataset for the forthcoming verification, analysis and functional analysis of barley PIF proteins.
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