Determination of PPARGC1 Cys430Ser polymorphism and MHS genotype in Croatian autochthonous pig breeds

Authors

  • Vladimir Margeta Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia
  • Polonca Frajman University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, 1230 Domžale, Slovenia
  • Gordana Kralik Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia
  • Peter Dovč University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, 1230 Domžale, Slovenia

Keywords:

PPARGC1, MHS, Black Slavonian pig, Turopolje pig, allele frequency

Abstract

The aim of the research was to determine PPARGC1 and MHS genetic status of two Croatian autochthonous pig breeds. The research was carried out on 20 Black Slavonian pigs and 20 Turopolje pigs. Genomic DNA was extracted from the blood. PCR was performed and PCR products genotyped by Restriction Fragment Length Polymorphism (RFLP). Allele frequency for PPRGC1 gene was determined by the Hardy-Weinberg equilibrium principle. Research results showed that A allele frequency in Black Slavonian pig was 44.44%, and of the T allele 55.56%. Homozygous genotypes (Ser/Ser) were dominant in Turopolje pig breeds (A = 100.00%). Determined MHS status proved both pig breeds to be completely MHS resistant. Our results point out the necessity to further investigate genetic status of both pig breeds in order to improve constitution and quality traits of muscle tissue in pure pig breeds.

Author Biography

  • Vladimir Margeta, Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek, Croatia

    corresponding author
    vmargeta@pfos.hr

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Published

2006-07-15

Issue

Vol. 10 No. 2 (2006): Acta Agraria Kaposváriensis

Section

Poster Section

License

Copyright (c) 2006 Vladimir Margeta, Polonca Frajman, Gordana Kralik, Peter Dovč

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Margeta, V., Frajman, P., Kralik, G., & Dovč, P. (2006). Determination of PPARGC1 Cys430Ser polymorphism and MHS genotype in Croatian autochthonous pig breeds. Acta Agraria Kaposváriensis, 10(2), 333-339. https://journal.uni-mate.hu/index.php/aak/article/view/1817

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