Set-up and comparison of dynamic simulation with different applications

Abstract

This paper examines the possibilities of building up dynamic simulations for the glycolysis of the
Saccharomyces Cerevisiae with two different applications. In one of the two applications it was
possible to set up the metabolic simulation in different resolutions and confront the results with the
published data. All the three simulation models supply adequate results like in tendencies and as
in dimensions, compared to the measured data. The linked models of individual enzyme-reactions
could result in the accumulation or run-out of intermediate components. This effect disappears if the
detailed model is built from elemental reactions. In this case a huge amount of kinetic and balance
parameter needs identification. The examined case shows well the difficulties of the clearing with
common components taking part in numerous cell processes, in a selected part-process. The main
advantage of the detailed metabolic model is that it contains direct enzyme and enzyme-complex
information. This allows the extension of the model with more real or theoretical reactions.