Examination of embryonic gonadal cell suspension integration in Hungarian landrace Guinea fowl
DOI:
https://doi.org/10.17205/SZIE.AWETH.2023.1.018Keywords:
Hungarian landrace guinea fowl, gene preservation, PGC, gonadAbstract
The present tendencies in animal husbandry put more and more emphasis on the gene preservation of the breeds excluded from production. While the mammalian species already have reliable methods for this, avians are problematic due to the homogametic nature of the male (ZZ) animals. To solve this problem the development of new technologies is necessary. In our experiment 10-day-old Hungarian landrace guinea fowl embryonic gonads were isolated and digested to create a cell suspension. This suspension contained various cell types, including primordial germ cells or PGCs. We marked the cells with the PKH26 red fluorescent stainer and injected them into the circulation of 3-day-old embryos. After four days we dissected the gonads from the 7-day-old embryos and examined the integration under stereo microscope according to the presence of stained cells. Our results showed that out of nine successful injections in three cases there were integrated stained cells in the gonads. Out of these in two cases female cells, in one case male cells were injected into the embryo. Our future goal is to include a freezing step into the experiment so we could check the potential of embryonic gonadal cell suspension injection in gene preservation.
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