In vitro binding of FITC-labelled plant lectins to gastrointestinal cells in the hen (Galius domesticus)

Authors

  • Károly Baintner Department of Physiology, Faculty of Animal Science, University of Kaposvár, H-7400 Kaposvár, Guba Sándor út 40. , Kaposvári Egyetem, Állattudományi Kar, Élettani Tanszék, 7400 Kaposvár, Guba Sándor út 40.
  • G. Jakab Department of Neurology, Uzsoki Hospital, Budapest, Uzsoki u. 29. , Uzsoki Kórház, Neurológiai Osztály, Budapest, Uzsoki u. 29.
  • Zs. Győri Department of Neurology, Uzsoki Hospital, Budapest, Uzsoki u. 29. , Uzsoki Kórház, Neurológiai Osztály, Budapest, Uzsoki u. 29.
  • P. Kiss Department of Agricultural Chemistry, University of Agriculture, H-2103 Gödöllő, Páter K. u. 1. , Agrártudományi Egyetem, Mezőgazdasági Kémia Tanszék, 2103 Gödöllő, Páter K. u. 1.

Keywords:

lectin, hen, avian, crop, stomach

Abstract

Binding of a panel of FITC-labelled lectins to deparaffinized histological sections from the crop, glandular stomach and duodenum of hens was studied. Succinyl-ConA produced a diffuse staining in the cytoplasm of epithelial cells in the three organs examined. In the epithelium of the crop, staining of the intercellular matrix by WGA, PHA, PNA and LCA displayed a net-like pattern. In the deep gastric glands, oxyntopeptic cells did not react with lectins except succinyl-ConA. Duodenal and gastric mucous cells were stained by WGA and PNA, but not by UEA-I or SNA-I. Binding of WGA and RPA-I to duodenal brush border was demonstrated. PHA did not attach to the brush border, which explains why this lectin is harmless if fed to chicken. The subepithelial connective tissue was strongly stained by LCA. The findings are discussed in relation to carbohydrate receptors and compared to the reactivity of mammalian tissues.

Author Biography

  • Károly Baintner, Department of Physiology, Faculty of Animal Science, University of Kaposvár, H-7400 Kaposvár, Guba Sándor út 40., Kaposvári Egyetem, Állattudományi Kar, Élettani Tanszék, 7400 Kaposvár, Guba Sándor út 40.

    corresponding author
    baintner@mail.atk.u-kaposvar.hu

References

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Published

2001-07-15

Issue

Vol. 5 No. 2 (2001): Acta Agraria Kaposváriensis

Section

Articles

License

Copyright (c) 2001 Baintner Károly, Jakab G., Győri Zs., Kiss P.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Baintner, K., Jakab, G., Győri, Z., & Kiss, P. (2001). In vitro binding of FITC-labelled plant lectins to gastrointestinal cells in the hen (Galius domesticus). Acta Agraria Kaposváriensis, 5(2), 47-53. https://journal.uni-mate.hu/index.php/aak/article/view/1578

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