Comparative Study of Laboratory Based Chlorophyll - A Determination Methods
DOI:
https://doi.org/10.33038/jcegi.3485Keywords:
chlorophyll-a concentration, chlorophyll-a determination methods, comperative studyAbstract
Protecting the quality and quantity of the available surface water resources is one of the most important points. Water quality is a complex concept that includes the physical, chemical, biological and hydrological parameters of water. One of the most significant biological water quality parameters is the chlorophyll-a content. Several different laboratory and field measurement methods are used to determine the chlorophyll-a content of water samples. The most frequently used laboratory methods are the Felföldy 1987 and the ISO 10260:1992 methods. Among field determination methods, submersible probes are frequently used to measure the chlorophyll-a concentration. Previously mentioned methods are based on fluorescence techniques. The main aim of this recent study is to compare different laboratory chlorophyll-a determination methods. Among the applied approaches, the Felföldy 1987 method and the ISO 10260:1992 method were compared. Furthermore, we measured the effect of the different solvent on the determination methods. Based on this, we investigated the Felföldy determination method using different solvents (methanol substituted with ethanol). The measurements were carried out under the same conditions from samples of Chlorella vulgaris culture in three repetitions by preparing a dilution series.
References
ÁCS, É. – BARRETO, S. – BESZTERI, B. – BORICS, G. – FEHÉR, G. – GRIGORSZKY, I. – KISS, K.T. – MAKK, J. – PELYHE, T. – SCHMIDT, A. – SZABÓ, K. – VÖRÖS, L. (2004): Algalógiai praktikum, ELTE Eötvös Kiadó, 361p.
BÖBBI, B., – PÁPISTA, É. (1998): Methodical problems of chlorophyll contetn determination, 9 th Hungarian Algological Meeting, Gárdony, 17p.
BORICS, G. (2015): Felszíni vizek fitoplankton alapú ökológiai állapotértékelése, Debreceni Egyetem, 65p.
DERENBACH, J. – ASTHEIMER, H. – HANSEN, H. – LEACH, H. (1979): Vertical Microscale Distribution of Phytoplankton in Relation to the Thermocline. Marine Ecology Progress Series, 1, 187–193. DOI: https://doi.org/10.3354/meps001187
DOERFFER, R. – SCHILLER, H. (2007): The MERIS case 2 water algorithm, International Journal of Remote Sensing, 28(3–4), 517–535. DOI: https://doi.org/10.1080/01431160600821127
FELFÖLDY, L. (1987): Biological water qualification. Water Management Institute, 190 p.
GREGOR, J. – MARŠÁLEK, B. (2004): Freshwater phytoplankton quantification by chlorophyll-a: a comparative study of in vitro, in vivo and in situ methods. Water Research, 38(3), 517–522. DOI: http://doi.org/10.1016/j.watres.2003.10.033
KALF, J. (2002): Limnology, Inland Water Ecosystems. Upper Saddle River, N.J. Prentice Hall, 355 p. Source: https://livresbioapp.files.wordpress.com/2016/03/limnology-kalff.pdf
KISS, K.T. (1998). Bevezetés az algalógiába, ELTE Eötvös Kiadó, 283 p.
PADISÁK, J. (2005): Általános Limnológia, ELTE Eötvös Kiadó, 310p.
PORRA, R.J. – THOMOSON, W.A., – KRIEDMENMANN, P.E. (1989): Determiation of accurate extinction coefficients and simultaneous equations for assaying chlorophlls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biopysica Acta, 975, 384–394. DOI: https://doi.org/10.1016/S0005-2728(89)80347-0
REYNOLDS, C.S. (2006): The Ecology of Phytoplankton. Cambridge University Press, 251p. DOI: https://doi.org/10.1017/CBO9780511542145
WETZEL, R.G. (2001): Limnology: Lake and River Ecosystems. (3 rd edition). Academic Press, 1024 p.
MSZ ISO 10260:1993 Vízminőség. A biokémiai paraméterek mérése. Az a-klorofill-koncentráció spektrofotometriás meghatározása Water quality. Measurement of biochemical parameters. Spectrometric determination of the chlorophyll-a concentration.
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