Investigation of performic acid oxidation in case of thiol-containing amino acid enantiomers

Authors

  • Csilla Albert Sapientia – Hungarian University of Transylvania, Csíkszereda Campus, Csíkszereda, Szabadság tér 1.
  • Péter Sára University of Kaposvár, Faculty of Animal Science, H-7400 Kaposvár, Guba S. u. 40.
  • Katalin Lóki University of Kaposvár, Faculty of Animal Science, H-7400 Kaposvár, Guba S. u. 40.
  • Éva Vargáné Visi University of Kaposvár, Faculty of Animal Science, H-7400 Kaposvár, Guba S. u. 40.
  • Sz. Salamon Sapientia – Hungarian University of Transylvania, Csíkszereda Campus, Csíkszereda, Szabadság tér 1.
  • János Csapó Sapientia – Hungarian University of Transylvania, Csíkszereda Campus, Csíkszereda, Szabadság tér 1. , University of Kaposvár, Faculty of Animal Science, H-7400 Kaposvár, Guba S. u. 40.

Keywords:

performic acid oxidation, cysteine and methionine enantiomers, racemization

Abstract

Performic acid oxidation of cysteine and methionine resulting in the formation of cysteic acid and methionine-sulphon has been applied in order to avoid the loss of these two sulphur containing amino acids during the acidic hydrolysis of proteins that is necessary prior to amino acid analysis. The aim of the research was assigned by the increasing demand for the determination of the amount amino acid enantiomers: the applicability of performic acid oxidation was evaluated in this point of view. Racemization of L-cysteine and L-methionine was found not significant during oxidation with performic acid, therefore this process can be applied before hydrolysis during quantification of cysteine and methionine enantiomers. Additionally, the quantification of cysteic acid and methionine-sulphon enantiomers was accomplished in the form of their diastereoisomer derivatives via the development of a reversed phase high performance liquid chromatography method.

Author Biography

  • Csilla Albert, Sapientia – Hungarian University of Transylvania, Csíkszereda Campus, Csíkszereda, Szabadság tér 1.

    corresponding author
    albertcsilla@sapientia.siculorum.ro

References

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Varga-Visi. É., Terlaky-Balla, É., Pohn, G., Kametler, L., Csapó, J. (2000). RPHPLC determination of L- and D-Cystine and cysteine as cysteic acid. Chromatographia, 51. 325–327. https://doi.org/10.1007/BF02492828

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Published

2006-07-15

Issue

Vol. 10 No. 2 (2006): Acta Agraria Kaposváriensis

Section

Poster Section

License

Copyright (c) 2006 Albert Csilla, Sára Péter, Lóki Katalin, Vargáné Visi Éva, Salamon Sz., Csapó János

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Albert, C., Sára, P., Lóki, K., Vargáné Visi, Éva, Salamon, S., & Csapó, J. (2006). Investigation of performic acid oxidation in case of thiol-containing amino acid enantiomers. Acta Agraria Kaposváriensis, 10(2), 349-354. https://journal.uni-mate.hu/index.php/aak/article/view/1816

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