First Description of Apple Rubbery Wood Virus 2 in Hungary by Small RNA High Throughput Sequencing
Keywords:
apple rubbery wood virus 2, small RNA, HTS, RT-PCRAbstract
Apple (Malus domestica) is one of the most widely produced and economically important fruits in temperate regions worldwide. Recently novel negative-stranded RNA viruses have been identified in fruit trees, including pome fruit trees. Apple rubbery wood virus 2 (ARWV2) is one of them belonging to family Phenuiviridae in the order Bunyavirales. ARWV2 possess negative-sense single-stranded tripartite RNA genome small (S), medium (M) and large (L) segments. Apple rubbery wood infectious disease is characterized by unusual flexibility of twigs and smaller branches due to lack of rigidity caused by decreased lignification of xylem vessels and fibers in the symptomatic trees. In our work we detected and validated the presence ARWV2 first time in Hungary in asymptomatic apple trees via small RNA high-throughput sequencing (HTS), and detected its presence in different apple, pear and quince trees by a reverse-transcription PCR (RT-PCR) based survey.
References
Czotter, N., Molnár, J., Pesti, R., Demián, E., Baráth, D., Varga, T. and Várallyay, É. 2018. Use of siRNAs for Diagnosis of Viruses Associated to Woody Plants in Nurseries and Stock Collections. Methods Mol. Biol. 1746 115–130. https://doi.org/10.1007/978-1-4939-7683-6_9
Hou, W., Li, S. and Massart, S. 2020. Is there a “Biological Desert” with the discovery of new plant viruses? A retrospective analysis for new fruit tree viruses. Frontiers in Microbiology. 11 592816. https://doi.org/10.3389/fmicb.2020.592816
Jakovljevic, V., Otten, P., Berwarth, C. and Jelkmann, W. 2017. Analysis of the apple rubbery wood disease by next generation sequencing of total RNA. European Journal of Plant Pathology. 148 (3) 637–646. https://doi.org/10.1007/s10658-016-1119-z
Kuhn, J. H., Adkins, S., Alioto, D., Alkhovsky, S.V., Amarasinghe, G.K., Anthony, S. J., Avšič-Županc, T., Ayllón, M. A., Bahl, J., Balkema-Buschmann, A., Ballinger, M. J., Bartonička, T., Basler, C., Bavari, S., Beer, M., Bente, D. A., Bergeron, É., Bird, B. H., Blair, C., Blasdell, K. R., Bradfute, S. B., Breyta, R., Briese, T., Brown, P. A., Buchholz, U. J., Buchmeier, M. J., Bukreyev, A., Burt, F., Buzkan, N., Calisher, C. H., Cao, M., Casas, I., Chamberlain, J., Chandran, K., Charrel, R. N., Chen, B., Chiumenti, M., Choi, I.-R., Clegg, J. C. S., Crozier, I., da Graça, J. V., Dal Bó, E., Dávila, A. M. R., de la Torre, J. C., de Lamballerie, X., de Swart, R. L., Di Bello, P. L., Di Paola, N., Di Serio, F., Dietzgen, R. G., Digiaro, M., Dolja, V. V., Dolnik, O., Drebot, M. A., Drexler, J. F., Dürrwald, R., Dufkova, L., Dundon, W. G., Duprex, W. P., Dye, J. M., Easton, A. J., Ebihara, H., Elbeaino, T., Ergünay, K., Fernandes, J., Fooks, A. R., Formenty, P. B. H., Forth, L. F., Fouchier, R. A. M., Freitas-Astúa, J., Gago-Zachert, S., Gāo, G. F., García, M. L., García-Sastre, A., Garrison, A. R., Gbakima, A., Goldstein, T., Gonzalez, J.-P. J., Griffiths, A., Groschup, M. H., Günther, S., Guterres, A., Hall, R. A., Hammond, J., Hassan, M., Hepojoki, J., Hepojoki, S., Hetzel, U., Hewson, R., Hoffmann, B., Hongo, S., Höper, D., Horie, M., Hughes, H. R., Hyndman, T. H., Jambai, A., Jardim, R., Jiāng, D., Jin, Q. and Jonson, G. B. 2020. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology. 165 (12) 3023–3072. https://doi.org/10.1007/s00705-020-04731-2
Rott, M. E., Kesanakurti, P., Berwarth, C., Rast, H., Boyes, I., Phelan, J. and Jelkmann, W. 2018. Discovery of Negative-Sense RNA Viruses in Trees Infected with Apple Rubbery Wood Disease by Next-Generation Sequencing. Plant Disease. 102 (7) 1254–1263. https://doi.org/10.1094/PDIS-06-17-0851-RE
Várallyay, E., Přibylová, J., Galbacs, Z.N., Jahan, A., Varga, T., Špak, J., Lenz, O., Fránová, J., Sedlák, J. and Koloniuk, I. 2022. Detection of Apple Hammerhead Viroid, Apple Luteovirus 1 and Citrus Concave Gum-Associated Virus in Apple Propagation Materials and Orchards in the Czech Republic and Hungary. Viruses. 14 (11) 2347. https://doi.org/10.3390/v14112347
Wang, Y., Wang, Y., Wang, G., Li, Q., Zhang, Z., Li, L., Lv, Y., Yang, Z., Guo, J. and Hong, N. 2022. Molecular Characteristics and Incidence of Apple Rubbery Wood Virus 2 and Citrus Virus A Infecting Pear Trees in China. Viruses. 14 (3) 576. https://doi.org/10.3390/v14030576
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Almash Jahan, Várallyay Éva
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Cikkre a Creative Commons 4.0 standard licenc alábbi típusa vonatkozik: CC-BY-NC-ND-4.0. Ennek értelmében a mű szabadon másolható, terjeszthető, bemutatható és előadható, azonban nem használható fel kereskedelmi célokra (NC), továbbá nem módosítható és nem készíthető belőle átdolgozás, származékos mű (ND). A licenc alapján a szerző vagy a jogosult által meghatározott módon fel kell tüntetni a szerző nevét és a szerzői mű címét (BY).
