In vitro technikák a növényvédő szerek szemirritációs tulajdonságának vizsgálatára

Abstract

The in vivo Draize-test is one of the most criticized in vivo methods, because of the subjective nature of the clinical observations and injuries of the test animals. Nowadays, several alternative tests are available which can be partly or totally replaced the in vivo eye irritation testing depending on the circumstances. The EpiOcular™ model and the Isolated Chicken Eye Test (ICET) are part of these alternative methods. Four different pesticides were examined with these in vitro methods. The eye irritation potential of the test items in the EpiOcular™ model were predicted by measurement of their cytotoxic effect, as reflected in the MTT assay. In the Isolated Chicken Eye Test the eye irritation potential of the test item was predicted based on the combination of the three endpoints: corneal swelling, corneal opacity and fluorescein retention. The based on the determination of each endpoint was the differences between values of the base line measurement and the values of any observation time points after the posttreatment rinse. Each test items showed irritation potential in both models. Compared these results with the available information about in vivo data of the tested pesticides, all test items are corresponded to them.