A DNA based method to determine the origin of honey produced by Apis cerana
Keywords:
Apis cerana, ethnic honey, molecular marker, honey authenticity, adulterationAbstract
The value of a product is derived from various components, such as its functionality, availability on the market, origin, raw material, exclusivity; in case of food products not least taste and health effects. In this approach honey has an outstanding position because of its reputation in the food and health industry. The honey of the Asian honey bee is unique and of great value, and because of this it is often prone to adulteration. Asian honey is restricted to those countries where the Asian honey bee is endemic. Beekeeping with this species of bee in these areas is part of cultural tradition. The idea of this work is to design a fast and accurate detection method for adulteration, in the interest of preserving the value of the honey produced by the Asian honey bee (Apis cerana). The purpose of the study was to point out the genetic differences between two close honey bee species (A. cerana and A. mellifera) and to use mitochondrial loci to develop a molecular method to confirm the entomological origin of honey deriving from a Cerana apiary. The markers designed besides being suitable per se can be adopted as a part of a genetic analysis panel.
References
Ajibola, A., Chamunorwa, J.P. and Erlwanger, K.H. 2013. Dietary supplementation with natural honey promotes growth and health of male and female rats compared to cane syrup. Scientific Research and Essays. 8(14). 543–553. https://doi.org/10.5897/SRE12.659.
Bogdanov, S. 2009. Honey composition. In The honey book. 5. 1–10.
Druml, B. and Cichna-Markl, M. 2014. High resolution melting (HRM) analysis of DNA–Its role and potential in food analysis. Food Chemistry. 158. 245–254. https://doi.org/10.1016/j.foodchem.2014.02.111.
Everstine, K., Spink, J. and Kennedy, S. 2013. Economically motivated adulteration (EMA) of food: common characteristics of EMA incidents. Journal of Food Protection. 76(4). 723–35. https://doi.org/10.4315/0362-028X.JFP-12-399.
FAO 2001. STAN 12-1981. Norma del Codex para la miel. Codex Aliemntarius. FAO/OMS.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic acids symposium series, London. Information Retrieval Ltd., c1979-c2000., pp. 95–98.
He, X., Wang, W., Qin, Q., Zeng, Z., Zhang, S. and Barron, A.B. 2013. Assessment of flight activity and homing ability in Asian and European honey bee species, Apis cerana and Apis mellifera, measured with radio frequency tags. Apidologie. 44(1). 38–51. https://doi.org/10.1007/s13592-012-0156-7.
Hisashi, F. 2010. Profitable beekeeping with Apis cerana. Bees for Development. 94. 8–11.
Kek, S.P., Chin, N.L., Tan, S.W., Yusof, Y.A. and Chua, L.S. 2017. Molecular identification of honey entomological origin based on bee mitochondrial 16S rRNA and COI gene sequences. Food Control. 78. 150–159. https://doi.org/10.1016/j.foodcont.2017.02.025.
Laube, I., Hird, H., Brodmann, P., Ullmann, S., Schöne-Michling, M., Chisholm, J. and Broll, H. 2010. Development of primer and probe sets for the detection of plant species in honey. Food Chemistry. 118(4). 979–986. https://doi.org/10.1016/j.foodchem.2008.09.063.
Moore, J.C., Spink, J. and Lipp, M. 2012. Development and application of a database of food ingredient fraud and economically motivated adulteration from 1980 to 2010. Journal of Food Science. 77(4). 118–126. https://doi.org/10.1111/j.1750-3841.2012.02657.x.
Moškrič, A., Mole, K. and Prešern, J. 2020. EPIC markers of the genus Apis as diagnostic tools for detection of honey fraud. Food Control. 121. 107634. https://doi.org/10.1016/j.foodcont.2020.107634.
Partap, U. and Verma, L. 1998. Asian bees and beekeeping: Issues and initiatives. In: M. Matsuka, L. Verma, S. Wongsiri, K. Shrestha and U. Partap (Editors), Proceedings of the 4th Asian apiculture association international conference, Kathmandu.
Reuber, B. 2015. 21st century homestead: Beekeeping. Lulu. com, 252 pp.
Ruiz-Matute, A., Rodríguez-Sánchez, S., Sanz, M. and Martínez-Castro, I. 2010. Detection of adulterations of honey with high fructose syrups from inulin by GC analysis. Journal of Food Composition and Analysis. 23(3). 273–276. https://doi.org/10.1016/j.jfca.2009.10.004.
Samat, S., Kanyan Enchang, F., Nor Hussein, F. and Wan Ismail, W.I. 2017. Four-week consumption of Malaysian honey reduces excess weight gain and improves obesity-related parameters in high fat diet induced obese rats. Evidence-based complementary and alternative medicine 2017. doi: https://doi.org/10.1155/2017/1342150.
Shapiro, A., Mu, W., Roncal, C., Cheng, K.-Y., Johnson, R.J. and Scarpace, P.J. 2008. Fructoseinduced leptin resistance exacerbates weight gain in response to subsequent high-fat feeding. American journal of physiology-regulatory, integrative and comparative physiology. https://doi.org/10.1152/ajpregu.00195.2008.
Sickel, W., Ankenbrand, M. J., Grimmer, G., Holzschuh, A., Härtel, S., Lanzen, J., Steffan-Dewenter, I. and Keller A. 2015. Increased efficiency in identifying mixed pollen samples by meta-barcoding with a dual-indexing approach. BMC ecology. 15(1). 20. https://doi.org/10.1186/s12898-015-0051-y.
Soares, S., Amaral, J. S., Oliveira, M.B.P. and Mafra, I. 2017. A comprehensive review on the main honey authentication issues: Production and origin. Comprehensive Reviews in Food Science and Food Safety. 16(5). 1072–1100. https://doi.org/10.1111/1541-4337.12278.
Soares, S., Grazina, L., Mafra, I., Costa, J., Pinto, M.A., Pham Duc, H., Oliveira, M.B.P. and Amaral, J.S. 2018. Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication. Food Research International. 105. 686–693. https://doi.org/10.1016/j.foodres.2017.11.081.
Svečnjak, L., Bubalo, D., Baranović, G. and Novosel, H. 2015. Optimization of FTIR-ATR spectroscopy for botanical authentication of unifloral honey types and melissopalynological data prediction. European Food Research and Technology. 240(6). 1101–1115. https://doi.org/10.1007/s00217-015-2414-1.
Theisen-Jones, H. and Bienefeld, K. 2016. The Asian Honey Bee (Apis cerana) is Significantly in Decline. Bee World. 93(4). 90–97. https://doi.org/10.1080/0005772X.2017.1284973.
Utzeri, V.J., Schiavo, G., Ribani, A., Tinarelli, S., Bertolini, F., Bovo, S. and Fontanesi, L. 2018. Entomological signatures in honey: an environmental DNA metabarcoding approach can disclose information on plant-sucking insects in agricultural and forest landscapes. Scientific reports. 8(1). 1–13. https://doi.org/10.1038/s41598-018-27933-w.
Verma, L. 1990. Beekeeping in integrated mountain development: Economic and scientific perspectives. Oxford & IBH Pub. Co.
Won, S.R., Lee, D.C., Ko, S.H., Kim, J.W., Rhee, H.I. 2008. Honey major protein characterization and its application to adulteration detection. Food Research International. 41(10). 952–956. https://doi.org/10.1016/j.foodres.2008.07.014.
Won, S.R., Li, C.Y., Kim, J.W. and Rhee, H.I. 2009. Immunological characterization of honey major protein and its application. Food Chemistry. 113(4). 1334–1338. https://doi.org/10.1016/j.foodchem.2008.08.082.
Zhu, X., Li, S., Shan, Y., Zhang, Z., Li, G., Su, D. and Liu, F. 2010. Detection of adulterants such as sweeteners materials in honey using near-infrared spectroscopy and chemometrics. Journal of Food Engineering. 101(1). 92–97. https://doi.org/10.1016/j.jfoodeng.2010.06.014.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Szepesi Kinga, Heltai Botond, Kolics Éva, Mátyás Kinga, Kálmán Nikoletta, Taller János, Satoshi Miyazaki, Kolics Balázs
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Cikkre a Creative Commons 4.0 standard licenc alábbi típusa vonatkozik: CC-BY-NC-ND-4.0. Ennek értelmében a mű szabadon másolható, terjeszthető, bemutatható és előadható, azonban nem használható fel kereskedelmi célokra (NC), továbbá nem módosítható és nem készíthető belőle átdolgozás, származékos mű (ND). A licenc alapján a szerző vagy a jogosult által meghatározott módon fel kell tüntetni a szerző nevét és a szerzői mű címét (BY).
