Transcriptome analysis of an ochratoxin-A biodegrading bacteria

Abstract

Fighting against and decreasing the effect of mycotoxins is an emerging problem. Among post- harvest methods are physical, chemical, and biological ones. This study is focusing on the biological tools for minimalizing the harmful effect of the ochratoxin-A (OTA) occurring on crops and fodders. The bacteria Cupriavidus basilensis ÖR16 strain has very good ability to detoxify ochratoxin-A to phenylalanine and ochratoxin-alfa. In previous studies the degradation rate of the ÖR16 bacteria was over 98%. The whole genome sequencing was also performed by our group in 2012. During this research, the enzymes, and genes responsible for the OTA degradation were characterized via transcriptome analyses. 15 genes were identified, which could play role in the degradation of OTA. Testing and investigating these nominated genes and enzymes could lead for a prepared fodder additive, which can help in the elimination of the negative effects of OTA in the future.