Effect of fumonisin B1 mycotoxin on membrane fluidity of human and rabbit erythrocytes

Authors

  • Dóra Hafner
  • Péter Bogner
  • Veronika Rajli
  • Gábor Tornyos
  • Roland Pósa
  • Péter Horn
  • Melinda Kovács

Keywords:

fumonisin B1, membrane fluidity, erythrocytes, rabbit, pig

Abstract

The authors examined the membrane damaging effect of fumonisin B1 (FB1). Fist, Hb-free ghosts of erythrocytes were isolated from healthy rabbits, incubated with purified FB1 (10, 50 and 100 μM) for 1 and 4 hours, labelled with DPH (1,6-diphenyl-1,3,5-hexatriene), for determining the fluorescent anisotropy. Secondly, intact human red blood cells were incubated with purified FB1 (50 μM and 1 mM) for 7 and 24 hours, and extracellular (EC) K-ion concentration as a sensitive indicator of membrane damage was determined. Finally, rabbits were treated with 5 mg FB1/day/animal for 10 days. Ghost cells isolated from the treated animals were labelled with DPH, and the fluorescent anisotropy was determined. Increasing concentration of FB1 (10−50−100 μM) did not cause change in the fluorescent anisotropy of the ghosts isolated from healthy rabbits. After incubation of the intact human erythrocytes with 50 μM FB1 for 7 hrs, the EC Na+ and K+ concentrations were 147.5−149.2 mmol/L and 0.04−1.64 mmol/L, respectively. No significant difference attributable to the toxin was detected. Incubation with the same concentration of FB1 for 24 hrs resulted in a slight increase in the EC Na+ level (182.4−190.6 mmol/L), while the K+ level remained unchanged. Ghosts of the rabbits treated with FB1 showed similar anisotropy to those of untreated animals. It could be concluded that FB1 in the applied concentrations did not cause changes in the different physical-chemical parameters examined in human and rabbit erythrocytes’ membranes.

Published

2009-02-15

How to Cite

Effect of fumonisin B1 mycotoxin on membrane fluidity of human and rabbit erythrocytes. (2009). ACTA AGRARIA KAPOSVARIENSIS, 13(1), 27-35. https://journal.uni-mate.hu/index.php/aak/article/view/1937

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