Molecular characterization of k-casein allelic variants in Bulgarian buffalo breed

Authors

  • Barbara Beneduci Department of Animal Science, University of Padova, 35020 Legnaro (PD), Agripolis - viale dell’Università 16., Italy
  • I. Krastanov Department of Cattle Breeding and Technologies, Agricultural Institute, Stara Zagora, Bulgaria
  • F. Maretto Department of Animal Science, University of Padova, 35020 Legnaro (PD), Agripolis - viale dell’Università 16., Italy
  • N. Oblakov Free Researcher Consultant, Sofia, Bulgaria
  • Martino Cassandro Department of Animal Science, University of Padova, 35020 Legnaro (PD), Agripolis - viale dell’Università 16., Italy

Keywords:

k-casein, genotyping, Murrah Bulgarian buffalo

Abstract

This study aimed to analyze the genetic polymorphism at the k-CN (CSN3) locus in the Murrah Bulgarian buffalo breed. A 221 bp fragment of exon IV of CSN3 gene was amplified and sequenced to detect polymorphisms in 215 animals. Genotyping, by means of sequence alignments, revealed the presence of one polymorphism at codon 135 that occurs with a substitution of a Thr (ACC) of the A genetic variant for an Ile (ATC) of the B genetic variant, while codon 148 resulted monomorphic for the presence of an Ala residue (GCT). Additionally a silent mutation Thr-Thr (ACC/ACT) occurs at 136 codon position. Genotypes frequencies at codon 135 were 0.56 (AA), 0.40 (AB) and 0.04 (BB) being the gene frequencies 0.76 and 0.24 for the A and B allele respectively. No significant deviation from Hardy-Weinberg Equilibrium was observed.

Author Biography

  • Barbara Beneduci, Department of Animal Science, University of Padova, 35020 Legnaro (PD), Agripolis - viale dell’Università 16., Italy

    corresponding author
    barbara.beneduci@live.it

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Published

2010-10-15

Issue

Vol. 14 No. 2 (2010): Acta Agraria Kaposváriensis

Section

Section 4 Buffalo, Horse and Sheep Breeding

License

Copyright (c) 2010 Barbara Beneduci, I. Krastanov, F. Maretto, N. Oblakov, Martino Cassandro

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Beneduci, B., Krastanov, I., Maretto, F., Oblakov, N., & Cassandro, M. (2010). Molecular characterization of k-casein allelic variants in Bulgarian buffalo breed. Acta Agraria Kaposváriensis, 14(2), 169-172. https://journal.uni-mate.hu/index.php/aak/article/view/1994

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