Variation of genetic diversity over time in local Italian chicken breeds undergoing conservation
Keywords:
conservation, local chicken breeds, genetic diversity, microsatellitesAbstract
Aim of this study was to measure the variation of different genetic diversity measures in five Italian local chicken breeds over a four years period of conservation. A total of 413 individuals were chosen for the analysis among the animals born in 2002 (a) and in
2006 (b) and genotyped for 20 microsatellite markers. In the animals sampled after four years of conservation activities the loss of alleles occurred, but only for those which had lower frequencies, with a mean number of alleles equal to 7.75 ± 3.08 in the year 2002 and 4.64 ± 1.60 in the year 2006. During the four years of conservation from 2002 to 2006, all breeds showed a not significant decrease of observed heterozygosis, ranging from 2.8% for Robusta Maculata to a maximum of 8.4% for Padovana. Expected heterozygosis showed the same trend, decreasing of 1% for Robusta Lionata to a maximum of 15% for Robusta Maculata. A decrease in the inbreeding coefficient occurred from 2002 to 2006 for Pépoi, Ermellinata di Rovigo and Robusta Maculata breeds, probably due to the unleashing of within breed genetic structures attributable to the rotation of males among the conservation flocks. On the other hand, an increment of the same index was noticed for the Robusta Lionata and Padovana breeds, for which an increase of inbreeding is probably due to the annual selection of individuals for morphological and productive traits. Genetic structure analysis revealed that the best number of populations fitting the dataset was five. Individuals were so assigned to one of five groups, approximately corresponding to the breed of origin. This result shows how no evident genetic structures were detectable within breed, both for 2002 and 2006 individuals. However, an increase in the proportion of membership for each breed has occurred in the year 2006 compared to the data obtained in the year 2002. Results obtained are very useful for planning new strategies for the conservation scheme, including the choice of the animals, a more efficient organization of matings and for the creation of a new selection index based on the maintenance of the existing genetic variation.
References
Baumung, R., Simianer, H., Hoffman, I. (2004). Genetic diversity studies in farm animals. A survey. J. Anim. Breed. Genet., 121. 6. 361–373. https://doi.org/10.1111/j.1439-0388.2004.00479.x
Cheng, H. H., Levin, I., Vallejo, R., Khatib, H., Dodgson, J. B., Crittenden, L. B., Hillel, J. (1995). Development of a genetic map of the chicken with markers of high utility. Poult. Sci., 74. 11. 1855–1874. https://doi.org/10.3382/ps.0741855
Dalvit, C., De Marchi, M., Dal Zotto, R., Zanetti, E., Meuwissen, T., Cassandro, M. (2008). Genetic characterization of the Burlina cattle breed using microsatellite molecular markers. J. Anim. Breed. Genet., 125. 2. 137–144. https://doi.org/10.1111/j.1439-0388.2007.00707.x
Dalvit, C., De Marchi, M., Zanetti, E., Cassandro, M. (2009). Genetic variation and population structure of Italian native sheep breeds undergoing in situ conservation. J. Anim. Sci., 87. 12. 3837–3844. https://doi.org/10.2527/jas.2008-1682
De Marchi, M., Dalvit, C., Targhetta, C., Cassandro, M. (2006). Assessing genetic diversity in indigenous Veneto chicken breeds using AFLP markers. Anim. Genet., 37. 2. 101–105. https://doi.org/10.1111/j.1365-2052.2005.01390.x
De Marchi, M., Dalvit, C., Targhetta, C., Cassandro, M. (2005b). Assessing genetic variability in two ancient chicken breeds of Padova area. Ital. J. Anim. Sci., 4. Suppl. 3. 151–153. https://doi.org/10.4081/ijas.2005.3s.151
De Marchi, M., Cassandro, M., Targhetta, C., Baruchello, M., Notter, D. R. (2005a). Conservation of poultry genetic resource in the Veneto region of Italy. Anim. Genet. Res. Inf., 37. 63–74. https://doi.org/10.1017/S1014233900001978
FAO (2007). The State of the World’s Animal Genetic Resources for Food and Agriculture. Commission on Genetic Resources for Food and Agriculture. Food and Agriculture Organization of the United Nations, Rome, Italy.
FAO Standing Committee (2004). Secondary Guidelines for Development of National Farm Animal Genetic Resources Management Plans. Measurement of Domestic Animal Diversity (Mo-DAD): Recommended Microsatellite Markers. http://dad.fao.org
Glowatzki-Mullis, M., Muntwyler, J., Baumle, E., Gaillard, C. (2008). Genetic diversity measures of Swiss goat breeds as decision-making support for conservation policy. Small Rumin. Res., 74. 1–3. 202–211. https://doi.org/10.1016/j.smallrumres.2007.07.002
Granevitze, Z., Hillel, J., Chen, G. H., Cuc, N. T. K., Feldman, M., Eding, H., Weigend, S. (2007). Genetic diversity within chicken populations from different continents and management histories. Anim. Genet., 38. 6. 576–583. https://doi.org/10.1111/j.1365-2052.2007.01650.x
Gutiérrez, J. P., Royo, L. J., Álvarez, I., Goyache, F. (2005). MolKin v3.0: a computer program for genetic analysis of populations using molecular coancestry information. J. Hered., 96. 6. 718–721. https://doi.org/10.1093/jhered/esi118
Hillel, J., Groenen, M. A. M., Tixier Boichard, M., Korol, A. B., David, L., Kirzhner, V. M., Burke, T., Barre Dirie, A., Crooijmans, R. P. M. A., Elo, K., Feldman, M. W., Freidlin, P. J., Maki-Tanila, A., Oortwijn, M., Thomson, P., Vignal, A., Wimmers, K., Weigend, S. (2003). Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools. Genet. Sel. Evol., 35. 533–557. https://doi.org/10.1186/1297-9686-35-6-533
Jordana, J., Folch, P., Aranguren, J. A. (2001). Microsatellites analysis of genetic diversity in the Catalonian donkey breed. J. Anim. Breed. Genet., 118. 57–63.
Marletta, D., Tupac-Yupanqui, I., Bordonaro, S., Garcia, D., Guastella, A. M., Criscione, A., Canon, J., Dunner, S. (2006). Analysis of genetic diversity and the determination of relationships among western mediterranean horse breeds using microsatellite markers. J. Anim. Breed. Genet., 123. 5. 315–325. https://doi.org/10.1111/j.1439-0388.2006.00603.x
Muchadeyi, F. C., Eding, H., Wollny, C. B. A., Groeneveld, E., Makuza, S. M., Shamseldin, R., Simianer, H., Weigend, S. (2007). Absence of population substructuring in Zimbabwe chicken ecotypes inferred using microsatellite analysis. Anim. Genet., 38. 4. 332–339. https://doi.org/10.1111/j.1365-2052.2007.01606.x
Pritchard, J. K., Stephens, M., Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics. 155. 2. 945–959. https://doi.org/10.1093/genetics/155.2.945
Raymond, M., Rousset, F. (1995). Genepop v. 3.1d. J. Hered., 86. 3. 248–249. https://doi.org/10.1093/oxfordjournals.jhered.a111573
SAS (1997). SAS Institute: SAS/STAT® User’s Guide: Statistics, Version 6.12, SAS Institute Inc., Cary, NC.
Takahashi, H., Nirasawa, K., Nagamine, Y., Tsudzuki, M., Yamamoto, Y. (1998). Genetic relationships among Japanese native breeds of chicken based on microsatellite DNA polymorphisms. J. Hered., 89. 543–546. https://doi.org/10.1093/jhered/89.6.543
Zanetti, E., De Marchi, M., Dalvit, C., Cassandro, M. (2010). Genetic characterization of local Italian breeds of chickens undergoing in situ conservation. Poult. Sci., 89. 3. 420–427. https://doi.org/10.3382/ps.2009-00324
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Copyright (c) 2010 Miriam Abbadi, Enrico Zanetti, Martino Cassandro
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