Effect of the longer equilibrations time for the membrane integrity of boar spermatozoa
Keywords:
semen for fertilization, semen deep freezing, longer equilibration, live and motility, tail stainigAbstract
Several laboratories make experiments with freezing of sperm of various livestock. The aim of our present work was to examine the effect of the extension of equilibration time before freezing on the membrane integrity of boar spermatozoa. In pursuance of our work, ejaculates of 7 boars were taken and the samples were cryopreserved by two freezing methods. One of the methods has one our longer equilibration time than the other one. After treatments before freezing and after warming the sperm, samples were taken and thereafter were stained by the method of Kovács–Foote. Afterwards the motility and the integrity of the acrosome were examined, and the two treatments were compared. To examine possible seasonal differences the experiments were done in summer and in autumn, too. The longer time of equilibration has positive effect on the viability of spermatozoa and the rate of live spermatozoa with intact acrosome was significantly higher in the longer equilibration group than for the control (P<0.05), and the average motility results of the two seasons show significant difference. The difference between so me individuals was not significant. We also didn’t find significant difference between the results of the summer and autumn experiments. Now, the treated sperms are stored in liquid nitrogen. We plan to make inseminations with these sperms in the future to control the suitability of both freezing methods.
References
Bamba, K., Cran, D. G. (1992). Effects of treatment with butylated hydroxytoluene on the susceptibility of boar spermatozoa to cold stress and dilution. J. Reprod. Fertil., 95(1), 69–77. https://doi.org/10.1530/jrf.0.0950069
Benson, R. W., Pickett, B. W., Komarek, R. J., Lucas, J. J. (1967). Effect of incubation and cold shock on motility of boar spermatozoa and their relationship to lipid content. J. Anim. Sci., 26(5), 1078–1081. https://doi.org/10.2527/jas1967.2651078x
Canvin, A. T., Buhr, M. M. (1989). Effect of temperature on the fluidity of boar spermatozoa membranes. J. Reprod. Fert., 85(2), 533–540. https://doi.org/10.1530/jrf.0.0850533
Crabo, B. G., Einarsson, S. (1971). Fertility of deep frozen boar semen. Acta. Vet. Scand., 12. 125–127.
Ewert, L. (1988). Experiments on preparation of boar spermatozoa for cryoconservation in straws and biological-physical aspects of thawing by microwaves. Thesis, School of Vet. Med., Hannover, 91.
Flowers, W. L. (1997). Management of boars for efficient semen production. J. Reprod. Fert., 52. 67–78.
Gadea, J., Matas, C. (2000). Sperm factors related to in vitro penetration of porcine oocytes. Theriogenology, 54(9), 1343–1357. https://doi.org/10.1016/S0093-691X(00)00458-1
Gadea, J., Sellés, E., Marco, M. A., Coy, P., Matás, C., Romar, R., Ruiz, S. (2004). Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62(3–4), 690–701. https://doi.org/10.1016/j.theriogenology.2003.11.013
Golyshev, N. A. (1985). Improvement of freezing technology of boar semen, Zhivotnovodstvo, 7. 49–51.
Graham, J. K., Hammerstedt, R. H. (1992). Differential effects of butylated hydroxytoluene analogs on bull sperm subjected to cool-induced membrane stress. Cryobiology, 29(1), 106–107. https://doi.org/10.1016/0011-2240(92)90010-Y
Graham, E. F., Rajamannan, A. H. J., Schmehl, M. K. L., Maki-Laurila, M., Bower, R. E. (1971). Fertility studies with frozen boar spermatozoa. A.I. Digest., 19. 16–18.
Ivanova, M., Mollova, M. (1993). Zona-penetration in vitro test for evaluating boar sperm fertility. Theriogenology, 40(2), 391–410. https://doi.org/10.1016/0093-691X(93)90277-C
Johnson, L. A., Weitze, K. F., Fiser, P., Maxwell, W. M. C. (2000). Storage of boar semen. Anim. Repr. Sci., 62(1–3), 143–172. https://doi.org/10.1016/S0378-4320(00)00157-3
Koehler, J. K. (1985). Mammalian sperm membranes: Their mosaic form and differential sensitivities. In: Deep freezing of boar semen. (Eds: Johnson, L. A., Larsson, K.) Uppsala, Sweden, 37–60.
Kovács, A., Foote, R. H. (1992). Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotechnic and Histochemistry, 67(3), 119–124. https://doi.org/10.3109/10520299209110020
Larsson, K. (1985). Boar sperm viability after freezing and thawing. Proc 1st Int. Conf. Deep Freezing of Boar Semen, Uppsala. 177–187.
Larsson, K., Einarsson, S. (1976a). Fertility of deep frozen boar spermatozoa. lnfluence of thaw diluents and of boars. Acta Vet. Scand., 17. 43–62. https://doi.org/10.1186/BF03547942
Larsson, K., Einarsson, S. (1976b). Influence of boars on the relationship between fertility and post-thawing sperm quality of deep frozen boar spermatozoa. Acta Vet Scand.,17. 74–82. https://doi.org/10.1186/BF03547944
Nagy, Sz., Házas, G., Bali Papp, Á., Iváncsics, J., Szász, F., Szász, F. Jr., Kovács, A. Foote, R. H. (1999). Evaluation of sperm tail membrane integrity by light microscopy. Theriogenology, 52(7), 1153–1159. https://doi.org/10.1016/S0093-691X(99)00207-1
Nagy, Sz., Hallap, T., Johannisson, A., Rodriguez-Martinez, H. (2004). Changes in plasma membrane and acrosome integrity of frozen-thawed bovine spermatozoa during a 4 h incubation as measured by multicolor flow cytometry. Anim. Reprod. Sci., 80(3–4), 225–235. https://doi.org/10.1016/j.anireprosci.2003.08.003
Paquignon, M., Courot, M. (1976). Fertilizing capacity of frozen boar spermatozoa. Proc. VIIIth Int. Congr. Anim. Reprod. & A.I, Cracow, 4. 1041–1044.
Paulenz, H., Drevle, I. S., Andersen, Berg K., Thomassen, R. (1995). The use of a dichromatic stain method (Spermac ) for determinating changes in the acrosomal integrity of boar semen during cryopreservation. Reprod. Dom. Anim., 30(3), 113–116. https://doi.org/10.1111/j.1439-0531.1995.tb00615.x
Pavelko, M. K., Crabo, B. G. (1976). Possible importance of some sperm coating proteins and their behaviour during preservation of boar spermatozoa. Proc. VIIIth Int. Congr. Anim. Reprod. & A. I., Cracow, 4. 1045–1048.
Polge, C., Salamon, S., Wilmut, I. (1970). Fertilizing capacity of frozen boar semen following surgical insemination. Vet. Rec., 87. 424–428.
Pursel, V. G., Johnson, L. A. (1971). Fertilizing capacity of frozen boar spermatozoa. J. Anim. Sci., 33. 1162.
Pursel, V. G., Johnson, S. A., Shulman, L. L. (1973). Effect of dilution, seminal plasma and incubation period on cold shock susceptibility of boar spermatozoa. J. Anim. Sci., 37(2), 532–535. https://doi.org/10.2527/jas1973.372528x
Pursel, V. G., Schulman, L. L., Johnson, L. A. (1978a). Effect of Orvus ES Paste on acrosome morphology, motility and fertilizing capacity of frozen-thawed boar sperm. J. Anim. Sci., 47(1), 198–202. https://doi.org/10.2527/jas1978.471198x
Pursel, V. G., Schulman, L. L., Johnson, L. A. (1978b). Distribution and morphology of fresh and frozen-thawed sperm in the reproductive tract of gilts after artificial insemination. Biol Reprod., 19(1), 69–76. https://doi.org/10.1095/biolreprod19.1.69
Pursel, V. G., Johnson, L. A. (1975). Freezing of boar spermatozoa. Fertilizing capacity with concentrated semen and a new thawning procedure. J. Anim. Sci., 40(1), 99–102. https://doi.org/10.2527/jas1975.40199x
Rodriguez-Martinez, H., Eriksson, B., Lundeheim, I. (1996). Freezing boar semen in flat plastic bags. Membrane integrity and fertility. Proc. 3rd Int. Conf. Deep Freezing Boar Semen. Reprod. Dom. Anim. 31(1), 161–168. https://doi.org/10.1111/j.1439-0531.1995.tb00020.x
Strzezek, J., Glogowski, J., Magierka, E., Lubera, Z., Jabbonosvska, C. (1984). Some aspects of cryobiochemistry of boar semen. Proc. 10th Int. Congr. Anim. Reprod. A.I., Urbana, IL 2. 224.
Tuli, R. K., Schmidt-Baulain, R., Holtz, W. (1992). Computer-assisted motility assessment of spermatozoa from fresh and frozen-thawed semen of the bull, boar and goat. Theriogenology, 38(3), 487–490. https://doi.org/10.1016/0093-691X(92)90068-3
Vazquez, J. M., Martinez, E., Roca, J., Lucas, X., Parrilla, I., Gil, M. A. (1999). Metodos y estrategias en la evaluacion de espermatozoides criopreservados de verraco. Proc. of II Congreso Iberico de Reproduccion Animal, 1. 377–381.
Verheyen, G., Pletincx, I., VanSteirteghem, A. (1993). Effect of freezing method, thawing temperature and post-thaw dilution/washing on motility (CASA) and morphology characteristics of high-quality human sperm. Hum. Reprod., 8(10), 1678–1684. https://doi.org/10.1093/oxfordjournals.humrep.a137912
Visser, D., Salamon, S. (1974). Effect of composition of Tris-based diluent on survival of boar spermatozoa following deep freezing. Aust. J. Biol. Sci., 27(5), 485–497. https://doi.org/10.1071/BI9740485
Weitze, K. F., Rath, D., Leps, H. (1988). Influenee of volume/surface ratio of plastic packages upon freeze-thaw rate and fertility of boar semen. Proc. 11th Int. Cong. Anim. Reprod. Artif. Insem., Dublin, 3. 312.
Woelders, H. (1990). Overview of in vitro methods for evaluation of semen quality. Reprod. Dom. Anim., 1. 146–165.
Westendorf, P., Richter, L., Treu, H. (1975). Zur Tiefgefrierung von Ebersperma Labor- und Besamungsergebnisse mit dem Hulsenberger Pailletten-Verfahren. Dtsch. Tierärtzl. Wschr., 82. 261–300.
Xu, X., Pommier, S., Arbov, T., Hutchings, B., Sootto, W., Foxcroft, G. R. (1998). In vitro maturation and fertilization techniques for assessment of semen quality and boar fertility. J. Anim. Sci., 76(12), 3079–3089. https://doi.org/10.2527/1998.76123079x
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Copyright (c) 2007 Makkosné Petz Brigitta, Pécsi T., Salamon I., Koltai Judit, Kiss R., Bali Papp Ágnes
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